Requirements for the design and production of high numerical aperture objectives for 4Pi confocal microscopy

被引:2
|
作者
Sure, T [1 ]
Wesner, J [1 ]
Heil, J [1 ]
机构
[1] Leica Microsyst GmbH, D-35578 Wetzlar, Germany
关键词
4Pi confocal microscopy; geometrical aberrations; chromatic aberrations; diffraction limited objectives; scalar diffraction theory; apodization; depolarization; Twyman-Green interferometer;
D O I
10.1117/12.449568
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We discuss the requirements on design and production regarding geometric and chromatic aberrations for objectives used in 4Pi confocal microscopy. We show that even the selection of a category 1/1 glass will not automatically assure that these requirements are met. due to residual variations in the Abbe number nu within the manufacturers tolerances. Consequently, the optical design has to take into consideration the possibility of balancing chromatic aberrations by varying selected air spacings in the final assembly of each individual objective. We also demonstrate. that for analyzing the influence of aberrations on the intensity distribution along the optical axis. a scalar diffraction theory is still applicable and very useful.
引用
收藏
页码:43 / 52
页数:10
相关论文
共 50 条
  • [1] CONFOCAL MICROSCOPY WITH AN INCREASED DETECTION APERTURE - TYPE-B 4PI CONFOCAL MICROSCOPY
    HELL, SW
    STELZER, EHK
    LINDEK, S
    CREMER, C
    [J]. OPTICS LETTERS, 1994, 19 (03) : 222 - 224
  • [2] 4Pi confocal microscopy with alternate interference
    Hell, SW
    Nagorni, M
    [J]. OPTICS LETTERS, 1998, 23 (20) : 1567 - 1569
  • [3] Design of high aperture confocal diffractive objectives
    Greysukh, Grigoriy Isaevitch
    Ezhov, Eugeniy Grigorievich
    Levin, Il’ya Anatolievich
    Stepanov, Sergei Alekseevich
    [J]. Computer Optics, 2011, 35 (01) : 22 - 28
  • [4] Aberrations in 4Pi Microscopy
    Hao, Xiang
    Antonello, Jacopo
    Allgeyer, Edward S.
    Bewersdorf, Joerg
    Booth, Martin J.
    [J]. OPTICS EXPRESS, 2017, 25 (13): : 14049 - 14058
  • [5] Confocal microscopy with a high numerical aperture parabolic mirror
    Drechsler, A
    Lieb, MA
    Debus, C
    Meixner, AJ
    Tarrach, G
    [J]. OPTICS EXPRESS, 2001, 9 (12): : 637 - 644
  • [6] PROPERTIES OF A 4PI CONFOCAL FLUORESCENCE MICROSCOPE
    HELL, S
    STELZER, EHK
    [J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1992, 9 (12) : 2159 - 2166
  • [7] 4Pi microscopy with negligible sidelobes
    Lang, M. C.
    Staudt, T.
    Engelhardt, J.
    Hell, S. W.
    [J]. NEW JOURNAL OF PHYSICS, 2008, 10
  • [8] 3-D TRANSFER-FUNCTION DESCRIPTION FOR 4PI CONFOCAL MICROSCOPY
    GU, M
    SHEPPARD, CJR
    [J]. ZOOLOGICAL STUDIES, 1995, 34 : 96 - 98
  • [9] Taylor series expansion based multidimensional image reconstruction for confocal and 4pi microscopy
    Dilipkumar, Shilpa
    Mondal, Partha Pratim
    [J]. APPLIED PHYSICS LETTERS, 2013, 103 (07)
  • [10] Polarization effects in 4Pi confocal microscopy studied with water-immersion lenses
    Bahlmann, K
    Hell, SW
    [J]. APPLIED OPTICS, 2000, 39 (10) : 1652 - 1658