Current analytical methods for porcine identification in meat and meat products

被引:72
|
作者
Zia, Qamar [1 ]
Alawami, Mohammad [1 ,2 ]
Mokhtar, Nur Fadhilah Khairil [3 ]
Nhari, Raja Mohd Hafidz Raja [3 ]
Hanish, Irwan [3 ,4 ]
机构
[1] A New Mind, Ash Shati 326173732, Al Qatif, Saudi Arabia
[2] Univ Cambridge, Depaartment Chem Engn & Biotechnol, Cambridge CB3 0AS, England
[3] Univ Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
[4] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Upm Serdang 43400, Selangor, Malaysia
关键词
Meat adulteration; ELISA; Species specific PCR; Nanobiosensor; DNA barcoding; Mass spectrometry; Digital PCR; Loop-mediated isothermal amplification (LAMP); Electronic noses; Real time PCR; REAL-TIME PCR; MEDIATED ISOTHERMAL AMPLIFICATION; LINKED-IMMUNOSORBENT-ASSAY; CLASS-MODELING TECHNIQUES; LIGHT-CHAIN ISOFORMS; SPECIES IDENTIFICATION; PORK ADULTERATION; PROCESSED MEAT; MULTIPLEX PCR; MASS-SPECTROMETRY;
D O I
10.1016/j.foodchem.2020.126664
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Authentication of meat products is critical in the food industry. Meat adulteration may lead to religious apprehensions, financial gain and food-toxicities such as meat allergies. Thus, empirical validation of the quality and constituents of meat is paramount. Various analytical methods often based on protein or DNA measurements are utilized to identify meat species. Protein-based methods, including electrophoretic and immunological techniques, are at times unsuitable for discriminating closely related species. Most of these methods have been replaced by more accurate and sensitive detection methods, such as DNA-based techniques. Emerging technologies like DNA barcoding and mass spectrometry are still in their infancy when it comes to their utilization in meat detection. Gold nanobiosensors have shown some promise in this regard. However, its applicability in small scale industries is distant. This article comprehensively reviews the recent developments in the field of analytical methods used for porcine identification.
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页数:29
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