Inhibition of calcium influx during hypoxia/reoxygenation in primary cultured rat hepatocytes

被引：11
|
作者：
Crenesse, D
Hugues, M
Ferre, C
Poiree, JC
Benoliel, J
Dolisi, C
Gugenheim, J
机构：
[1] Fac Med Nice, Physiol Lab, F-06107 Nice 2, France
[2] Univ Bordeaux 2, UFR Pharm, Lab Physiol Cellulaire & Pharmacol Mol, CNRS ESA 5017, F-33076 Bordeaux, France
[3] Fac Med Nice, Chirurg Expt Lab, Nice, France
[4] Fac Med Nice, Biochim Lab, Nice, France
[5] Fac Med Nice, Biophys Lab, Nice, France
来源：
PHARMACOLOGY | 1999年 / 58卷 / 03期
关键词：
calcium influx; hypoxia/reoxygenation; hepatocytes; rat;
D O I：
10.1159/000028278
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Calcium has been demonstrated to play an important role in hepatocyte damage during ischemia/reperfusion phases. Calcium influx was determined in primary cultured rat hepatocytes submitted to a succession of warm hypoxia and reoxygenation phases in the presence of diltiazem, gallopamil and a Na+/ H+ antiport inhibitor, HOE-694:. Only diltiazem significantly inhibited calcium influx with higher potency after reoxygenation than after hypoxia only, suggesting a complex mechanism of action of diltiazem which could act on different physiological functions involved in Ca2+ invasion of hepatocytes after hypoxic insult.
引用
收藏
页码：160 / 170
页数：11
相关论文
共 50 条
  • [1] Calcium influx during hypoxia/reoxygenation in primary cultured rat hepatocytes: Protective effect of University of Wisconsin solution
    Crenesse, D
    Hugues, M
    Ferre, C
    Poiree, JC
    Dolisi, C
    Gugenheim, J
    CRYO-LETTERS, 1997, 18 (03) : 143 - 152
  • [2] HYPOXIA AND REOXYGENATION INJURY IN RAT HEPATOCYTES
    MURDOCK, A
    TAN, S
    RIVERA, A
    PARKS, D
    CLINICAL RESEARCH, 1991, 39 (04): : A805 - A805
  • [3] Mapacalcine specifically blocks hypoxia-induced calcium influx in rat hepatocytes
    Crenesse, D
    Neuilly, G
    Gugenheim, J
    Ferre, C
    Hugues, M
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (09): : 1952 - 1957
  • [4] Effects of Wy14643 on hypoxia/reoxygenation injury in rat primary hepatocytes
    Chen, Ke
    Li, Yuanhai
    Xu, Siqi
    Hu, Shenhong
    ADVANCES ON MEDICAL SCIENCES: PROCEEDINGS OF THE 14TH CONFERENCE ON ANAESTHESIOLOGY, 2009, 2009, : 43 - 56
  • [5] Effect of hypoxia and reoxygenation on metabolic pathways in rat hepatocytes
    Villalobos-Molina, R
    Saavedra-Molina, A
    Devlin, TM
    ARCHIVES OF MEDICAL RESEARCH, 1998, 29 (03): : 219 - 223
  • [6] Alterations in the expression of the AQP family in cultured rat astrocytes during hypoxia and reoxygenation
    Yamamoto, N
    Yoneda, K
    Asai, K
    Sobue, K
    Tada, T
    Fujita, Y
    Katsuya, H
    Fujita, M
    Aihara, N
    Mase, M
    Yamada, K
    Miura, Y
    Kato, T
    MOLECULAR BRAIN RESEARCH, 2001, 90 (01): : 26 - 38
  • [7] JNK1/SAPK1 involvement in hypoxia-reoxygenation-induced apoptosis in primary cultured rat hepatocytes.
    Crenesse, D
    Schmid-Alliana, R
    Laurens, M
    Cursio, R
    Gugenheim, J
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2000, 440 (06): : R247 - R247
  • [8] COMPARATIVE SUSCEPTIBILITY OF CULTURED-HEPATOCYTES AND KUPFFER CELLS TO HYPOXIA AND HYPOXIA-REOXYGENATION
    SAMARASINGHE, DA
    FARRELL, GC
    HEPATOLOGY, 1994, 20 (04) : A395 - A395
  • [9] Calcium influx mechanisms underlying calcium oscillations in rat hepatocytes
    Jones, Bertina F.
    Boyles, Rebecca R.
    Hwang, Sung-Yong
    Bird, Gan S.
    Putney, James W.
    HEPATOLOGY, 2008, 48 (04) : 1273 - 1281
  • [10] Mitochondrial calcium transporting pathways during hypoxia and reoxygenation in single rat cardiomyocytes
    Griffiths, EJ
    Ocampo, CJ
    Savage, JS
    Rutter, GA
    Hansford, RG
    Stern, MD
    Silverman, HS
    CARDIOVASCULAR RESEARCH, 1998, 39 (02) : 423 - 433
12345