One step purification of glucose-6-phosphate dehydrogenase from brain areas by immunoaffinity chromatography

被引：1
|
作者：
Capellacci, S [1 ]
Aluigi, G [1 ]
Tabellini, L [1 ]
Ninfali, P [1 ]
机构：
[1] Ist Chim Biol G Fornaini, I-61029 Urbino, PS, Italy
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 05期
关键词：
brain cortex; glucose-6-phosphate dehydrogenase; immunoaffinity chromatography; N-terminal group; protein purification;
D O I：
10.1023/A:1005645321854
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucose-6-phosphate dehydrogenase was purified from rabbit brain cortex using a single immunoaffinity chromatographic step and was contaminated only by a 50 kDa protein. The proteins, separated by SDS-PAGE, were sequenced: the glucose-6-phosphate dehydrogenase was blocked at the N-terminal, the co-eluted protein was similar to alpha -tubulin. Our technique can be applied to purification and sequencing of the enzyme from brain areas or to measure its turnover rate in cultured cells.
引用
收藏
页码：353 / 357
页数：5
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