One step purification of glucose-6-phosphate dehydrogenase from brain areas by immunoaffinity chromatography

被引：1

作者：

Capellacci, S ^{[1
]}

Aluigi, G ^{[1
]}

Tabellini, L ^{[1
]}

Ninfali, P ^{[1
]}

机构：

[1] Ist Chim Biol G Fornaini, I-61029 Urbino, PS, Italy

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 05期

关键词：

brain cortex; glucose-6-phosphate dehydrogenase; immunoaffinity chromatography; N-terminal group; protein purification;

D O I：

10.1023/A:1005645321854

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucose-6-phosphate dehydrogenase was purified from rabbit brain cortex using a single immunoaffinity chromatographic step and was contaminated only by a 50 kDa protein. The proteins, separated by SDS-PAGE, were sequenced: the glucose-6-phosphate dehydrogenase was blocked at the N-terminal, the co-eluted protein was similar to alpha -tubulin. Our technique can be applied to purification and sequencing of the enzyme from brain areas or to measure its turnover rate in cultured cells.

引用

页码：353 / 357

页数：5

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