Cloning and expression of canine interferon-α genes in Escherichia coli

被引：14
|
作者：
Taira, O
Watanugi, I
Hagiwara, Y
Takahashi, M
Arai, S
Sato, H
Maehara, N
机构：
[1] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Vet Microbiol, Towada, Aomori 0348628, Japan
[2] Kitasato Inst, Kitamoto, Saitama 3640026, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2005年 / 67卷 / 10期
关键词：
canine IFN-alpha; cloning; expression;
D O I：
10.1292/jvms.67.1059
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We cloned five new subtypes of cDNA encoding canine interferon-a (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alpha s were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alpha s were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1, but not against canine herpesvirus-1.
引用
收藏
页码：1059 / 1062
页数：4
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