Cloning and expression of canine interferon-α genes in Escherichia coli

被引：14
|
作者：
Taira, O
Watanugi, I
Hagiwara, Y
Takahashi, M
Arai, S
Sato, H
Maehara, N
机构：
[1] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Vet Microbiol, Towada, Aomori 0348628, Japan
[2] Kitasato Inst, Kitamoto, Saitama 3640026, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2005年 / 67卷 / 10期
关键词：
canine IFN-alpha; cloning; expression;
D O I：
10.1292/jvms.67.1059
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We cloned five new subtypes of cDNA encoding canine interferon-a (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alpha s were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alpha s were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1, but not against canine herpesvirus-1.
引用
收藏
页码：1059 / 1062
页数：4
相关论文
共 50 条
  • [21] Soluble Expression, Rapid Purification and Antiviral Activity of Recimbinant Bovine Interferon-α in Escherichia coli
    Yu, H. -Y.
    Liu, J.
    He, Z. -Y.
    Zhou, W.
    Xia, B. -B.
    Wang, M.
    Chen, J.
    Wang, M. -L.
    Jiang, G. -T.
    Zhao, J.
    APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2020, 56 (02) : 154 - 163
  • [22] Cloning and expression of elta and eaea genes from porcine Escherichia coli (I)
    Vanghele, M
    Popa, V
    Cismileanu, A
    BULLETIN OF THE UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE, VOL 60: VETERINARY MEDICINE, 2003, 60 : 173 - 178
  • [23] CLONING AND EXPRESSION OF RHODOCOCCUS GENES ENCODING PIGMENT PRODUCTION IN ESCHERICHIA-COLI
    HILL, R
    HART, S
    ILLING, N
    KIRBY, R
    WOODS, DR
    JOURNAL OF GENERAL MICROBIOLOGY, 1989, 135 : 1507 - 1513
  • [24] CLONING, SEQUENCING, AND EXPRESSION OF THE ESCHERICHIA-COLI CYTOLETHAL DISTENDING TOXIN GENES
    PICKETT, CL
    COTTLE, DL
    PESCI, EC
    BIKAH, G
    INFECTION AND IMMUNITY, 1994, 62 (03) : 1046 - 1051
  • [25] PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli
    Daniel K.Y. Solaiman
    Biotechnology Letters, 2000, 22 : 789 - 794
  • [26] CLONING AND EXPRESSION OF 2 PASTEURELLA-MULTOCIDA GENES IN ESCHERICHIA-COLI
    MANOHA, F
    CHEVALIER, G
    WROBLEWSKI, H
    DELAMARCHE, C
    BIOCHIMIE, 1994, 76 (01) : 9 - 14
  • [27] PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli
    Solaiman, DKY
    BIOTECHNOLOGY LETTERS, 2000, 22 (09) : 789 - 794
  • [28] CLONING AND EXPRESSION OF SERRATIA CHITINASE GENES IN ESCHERICHIA-COLI AND PSEUDOMONAS RHIZOBACTERIA
    JONES, J
    ZIEGLE, JS
    SUSLOW, TV
    PHYTOPATHOLOGY, 1984, 74 (07) : 863 - 863
  • [29] CLONING AND EXPRESSION OF CLOSTRIDIUM-STERCORARIUM CELLULASE GENES IN ESCHERICHIA-COLI
    SCHWARZ, WH
    JAURIS, S
    KOUBA, M
    BRONNENMEIER, K
    STAUDENBAUER, WL
    BIOTECHNOLOGY LETTERS, 1989, 11 (07) : 461 - 466
  • [30] CLONING OF ESCHERICHIA-COLI GLYCOGEN GENES
    OKITA, TW
    PREISS, J
    RODRIGUIZ, RL
    FEDERATION PROCEEDINGS, 1980, 39 (06) : 2014 - 2014
12345