Separation of cephalosporin C acylase and penicillin V acylase from Aeromonas ACY 95 by chemical treatment

被引：4
|
作者：
Ambedkar, SS [1 ]
Deshpande, BS [1 ]
Shewale, JG [1 ]
机构：
[1] HINDUSTAN ANTIBIOT LTD, RES & DEV, PUNE 411018, MAHARASHTRA, INDIA
来源：
PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 04期
关键词：
cephalosporin C acylase; penicillin V acylase; Aeromonas; 7-aminocephalosporanic acid; 6-aminopenicillanic acid; chemical extraction;
D O I：
10.1016/S0032-9592(96)00059-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Several surfactants and solvents were evaluated for the extraction of cephalosporin C acylase from Aeromonas ACY 95. Treatment of the cells with acetone (0.5% v/v) allowed 85% recovery of cephalosporin C acylase with only 10% penicillin V acylase. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：305 / 307
页数：3
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