Separation of cephalosporin C acylase and penicillin V acylase from Aeromonas ACY 95 by chemical treatment

被引：4
|
作者：
Ambedkar, SS [1 ]
Deshpande, BS [1 ]
Shewale, JG [1 ]
机构：
[1] HINDUSTAN ANTIBIOT LTD, RES & DEV, PUNE 411018, MAHARASHTRA, INDIA
来源：
PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 04期
关键词：
cephalosporin C acylase; penicillin V acylase; Aeromonas; 7-aminocephalosporanic acid; 6-aminopenicillanic acid; chemical extraction;
D O I：
10.1016/S0032-9592(96)00059-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Several surfactants and solvents were evaluated for the extraction of cephalosporin C acylase from Aeromonas ACY 95. Treatment of the cells with acetone (0.5% v/v) allowed 85% recovery of cephalosporin C acylase with only 10% penicillin V acylase. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：305 / 307
页数：3
相关论文
共 50 条
  • [31] Computational design to improve catalytic activity of cephalosporin C acylase from Pseudomonas strain N176
    Tian, Ye
    Xu, Zhaobin
    Huang, Xiaoqiang
    Zhu, Yushan
    RSC ADVANCES, 2017, 7 (48): : 30370 - 30375
  • [32] THE HYDROLYSIS OF CEPHALOSPORIN-G BY FREE AND IMMOBILIZED PENICILLIN-G ACYLASE FROM A MUTANT OF ESCHERICHIA-COLI ATCC-11105
    ERARSLAN, A
    PROCESS BIOCHEMISTRY, 1993, 28 (05) : 311 - 318
  • [33] A thermal study on the use of immobilized penicillin G acylase in the formation of 7-amino-3-deacetoxy cephalosporanic acid from cephalosporin G
    Pan, JL
    Syu, MJ
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2004, 79 (10) : 1050 - 1056
  • [34] Computational design of variants for cephalosporin C acylase from Pseudomonas strain N176 with improved stability and activity
    Ye Tian
    Xiaoqiang Huang
    Qing Li
    Yushan Zhu
    Applied Microbiology and Biotechnology, 2017, 101 : 621 - 632
  • [35] Characterization of smallest active monomeric penicillin V acylase from new source:: A yeast, Rhodotorula aurantiaca (NCIM 3425)
    Kumar, Atul
    Prabhune, Asmita
    Suresh, C. G.
    Pundle, Archana
    PROCESS BIOCHEMISTRY, 2008, 43 (09) : 961 - 967
  • [36] Structural analysis of a penicillin V acylase from Pectobacterium atrosepticum confirms the importance of two Trp residues for activity and specificity
    Avinash, Vellore Sunder
    Panigrahi, Priyabrata
    Chand, Deepak
    Pundle, Archana
    Suresh, Cheravakattu Gopalan
    Ramasamy, Sureshkumar
    JOURNAL OF STRUCTURAL BIOLOGY, 2016, 193 (02) : 85 - 94
  • [37] Computational design of variants for cephalosporin C acylase from Pseudomonas strain N176 with improved stability and activity
    Tian, Ye
    Huang, Xiaoqiang
    Li, Qing
    Zhu, Yushan
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (02) : 621 - 632
  • [38] HIGH-LEVEL PRODUCTION, CHEMICAL MODIFICATION AND SITE-DIRECTED MUTAGENESIS OF A CEPHALOSPORIN-C ACYLASE FROM PSEUDOMONAS STRAIN-N176
    ISHII, Y
    SAITO, Y
    FUJIMURA, T
    SASAKI, H
    NOGUCHI, Y
    YAMADA, H
    NIWA, M
    SHIMOMURA, K
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 230 (02): : 773 - 778
  • [39] Kinetically controlled acylation of 6-APA catalyzed by penicillin acylase from Streptomyces lavendulae: effect of reaction conditions in the enzymatic synthesis of penicillin V
    Hormigo, Daniel
    Teresa Lopez-Conejo, Maria
    Serrano-Aguirre, Lara
    Garcia-Martin, Alberto
    Saborido, Ana
    de la Mata, Isabel
    Arroyo, Miguel
    BIOCATALYSIS AND BIOTRANSFORMATION, 2020, 38 (04) : 253 - 262
  • [40] CLONING AND NUCLEOTIDE SEQUENCING OF NEW GLUTARYL 7-ACA AND CEPHALOSPORIN-C ACYLASE GENES FROM PSEUDOMONAS STRAINS
    ARAMORI, I
    FUKAGAWA, M
    TSUMURA, M
    IWAMI, M
    ISOGAI, T
    ONO, H
    ISHITANI, Y
    KOJO, H
    KOHSAKA, M
    UEDA, Y
    IMANAKA, H
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 72 (04): : 232 - 243
12345