The calcium uniporter regulates the permeability transition pore in isolated cortical mitochondria

被引：14
|
作者：
Yu, Ning [1 ]
Wang, Shilei [1 ]
Wang, Peng [1 ]
Li, Yu [1 ]
Li, Shuhong [1 ]
Wang, Li [1 ]
Chen, Hongbing [2 ]
Wang, Yanting [1 ]
机构：
[1] Qingdao Univ Med Coll, Affiliated Hosp, Dept Anesthesiol, Qingdao 266003, Shandong, Peoples R China
[2] Qingdao Univ Med Coll, Cerebrovasc Dis Inst, Qingdao 266003, Shandong, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 02期
基金：
中国国家自然科学基金;
关键词：
mitochondria; calcium uniporter; permeability transition pore; neuron; cortex; cell death; cerebroprotection; DEPENDENT ANION CHANNEL; BRAIN MITOCHONDRIA; CELL-DEATH; REPERFUSION INJURY; CA2+ UNIPORTER; RAT-LIVER; APOPTOSIS; RELEASE; COMPLEX; INVOLVEMENT;
D O I：
10.3969/j.issn.1673-5374.2012.02.005
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
To investigate the influence of the mitochondrial calcium uniporter on the mitochondrial permeability transition pore, the present study observed mitochondrial morphology in cortical neurons isolated from adult rats using transmission electron microscopy, and confirmed the morphology and activity of isolated mitochondria by detecting succinic dehydrogenase and monoamine oxidase, two mitochondrial enzymes. Isolated mitochondria were treated with either ruthenium red, an inhibitor of the uniporter, spermine, an activator of the uniporter, or in combination with cyclosporin A, an inhibitor of the mitochondrial permeability transition pore. Results showed that ruthenium red inhibited CaCl2-induced mitochondrial permeability transition pore opening, spermine enhanced opening, and cyclosporin A attenuated the effects of spermine. Results demonstrated that the mitochondrial calcium uniporter plays a role in regulating the mitochondrial permeability transition pore in mitochondria isolated from the rat brain cortex.
引用
收藏
页码：109 / 113
页数：5
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