The calcium uniporter regulates the permeability transition pore in isolated cortical mitochondria

被引：14

作者：

Yu, Ning ^{[1
]}

Wang, Shilei ^{[1
]}

Wang, Peng ^{[1
]}

Li, Yu ^{[1
]}

Li, Shuhong ^{[1
]}

Wang, Li ^{[1
]}

Chen, Hongbing ^{[2
]}

Wang, Yanting ^{[1
]}

机构：

[1] Qingdao Univ Med Coll, Affiliated Hosp, Dept Anesthesiol, Qingdao 266003, Shandong, Peoples R China

[2] Qingdao Univ Med Coll, Cerebrovasc Dis Inst, Qingdao 266003, Shandong, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 02期

基金：

中国国家自然科学基金;

关键词：

mitochondria; calcium uniporter; permeability transition pore; neuron; cortex; cell death; cerebroprotection; DEPENDENT ANION CHANNEL; BRAIN MITOCHONDRIA; CELL-DEATH; REPERFUSION INJURY; CA2+ UNIPORTER; RAT-LIVER; APOPTOSIS; RELEASE; COMPLEX; INVOLVEMENT;

D O I：

10.3969/j.issn.1673-5374.2012.02.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To investigate the influence of the mitochondrial calcium uniporter on the mitochondrial permeability transition pore, the present study observed mitochondrial morphology in cortical neurons isolated from adult rats using transmission electron microscopy, and confirmed the morphology and activity of isolated mitochondria by detecting succinic dehydrogenase and monoamine oxidase, two mitochondrial enzymes. Isolated mitochondria were treated with either ruthenium red, an inhibitor of the uniporter, spermine, an activator of the uniporter, or in combination with cyclosporin A, an inhibitor of the mitochondrial permeability transition pore. Results showed that ruthenium red inhibited CaCl2-induced mitochondrial permeability transition pore opening, spermine enhanced opening, and cyclosporin A attenuated the effects of spermine. Results demonstrated that the mitochondrial calcium uniporter plays a role in regulating the mitochondrial permeability transition pore in mitochondria isolated from the rat brain cortex.

引用

页码：109 / 113

页数：5

共 50 条

[21] Does the Ca2+ uniporter form the mitochondrial permeability transition pore?
Brustovetsky, N
Dubinsky, JM
BIOPHYSICAL JOURNAL, 1998, 74 (02) : A384 - A384
[22] Aging enhances the activation of the permeability transition pore in mitochondria
Mather, M
Rottenberg, H
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 273 (02) : 603 - 608
[23] Mitochondria and the permeability transition pore in cancer metabolic reprogramming
Guo, Lishu
BIOCHEMICAL PHARMACOLOGY, 2021, 188
[24] Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
Marcu, Raluca
Neeley, Chris K.
Karamanlidis, Georgios
Hawkins, Brian J.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2012, (67):
[25] Reactive oxygen species-induced openings of the permeability transition pore in single isolated mitochondria
Hüser, J
Blatter, LA
BIOPHYSICAL JOURNAL, 1998, 74 (02) : A383 - A383
[26] Melatonin modulates permeability transition pore by inhibition of mitochondrial KATP channels in isolated brain mitochondria
Waseem, M.
Tabassum, H.
Parvez, S.
JOURNAL OF NEUROCHEMISTRY, 2017, 142 : 249 - 249
[27] Imaging the permeability transition in single isolated mitochondria
Hüser, J
Rechenmacher, CE
Blatter, LA
BIOPHYSICAL JOURNAL, 1998, 74 (02) : A18 - A18
[28] HAX-1 regulates cyclophilin-D levels and mitochondria permeability transition pore in the heart
Lam, Chi Keung
Zhao, Wen
Liu, Guan-Sheng
Cai, Wen-Feng
Gardner, George
Adly, George
Kranias, Evangelia G.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (47) : E6466 - E6475
[29] PHO-ERK1/2 interaction with mitochondria regulates the permeability transition pore in cardioprotective signaling
Hernandez-Resendiz, Sauri
Zazueta, Cecilia
LIFE SCIENCES, 2014, 108 (01) : 13 - 21
[30] Platelet Mitochondria Calcium Uniporter Regulates ITAM-Dependent Platelet Activation and Signaling
Gomez, Abigail Ajanel
Denorme, Frederik
Portier, Irina
Kowalczyk, Mia
Rameshbhatt, Sradha
Kosaka, Yasuhiro
Campbell, Robert A.
BLOOD, 2023, 142

← 1 2 3 4 5 →