Ginsenosides in hairy roots of a Panax hybrid

被引:20
|
作者
Washida, D
Shimomura, K
Nakajima, Y
Takido, M
Kitanaka, S
机构
[1] Nihon Univ, Coll Pharm, Funabashi, Chiba 274, Japan
[2] Natl Inst Hlth Sci, Tsukuba Med Plant Res Stn, Tsukuba, Ibaraki 305, Japan
[3] Nagano Agr Technique & Training Ctr, Nagano 384, Japan
关键词
Panax ginseng; Panax quinquefolium; Araliaceae; interspecific hybrid; hairy root culture; ginsenoside;
D O I
10.1016/S0031-9422(98)00308-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hairy roots of an interspecific hybrid ginseng (Panax ginseng x P. quinquefolium), named Pgq, were established by the infection of Agrobacterium rhizogenes ATCC 15834. Growth and ginsenosides content of hairy roots cultured in various basal liquid media were measured periodically from 2 to 8 weeks. In Gamborg B5 liquid medium, the hairy roots showed best growth (5.87 g fresh weight per flask) at week 8. The highest content of ginsenoside was 2.87% as dry weight at week 8 when cultured in 1/8 Murashige-Skoog liquid medium. The ginsenoside content of Pgq hairy roots was comparable to that of Pgq root cultivated in the field. However the highest yield of ginsenosides was obtained in B5 liquid medium (3.85 mg per flask at week 8). (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2331 / 2335
页数:5
相关论文
共 50 条
  • [41] The Effect of Growth Regulators on Quality Parameters and Ginsenosides Accumulation in Panax quinquefolium L. Roots
    Kołodziej Barbara
    Kochan Ewa
    Kazimierczak Jerzy
    Chmiel Aleksander
    Plant Growth Regulation, 2006, 48 : 13 - 19
  • [42] Foam separation of ginsenosides of panax ginseng
    Xiu, Z.L.
    Zhang, D.J.
    Jia, L.Y.
    Fan, J.
    Zhang, Y.W.
    Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering, 2001, 1 (03):
  • [43] High Levels of Ginsenosides Production Associated with roIC Gene Transcription and Expression in Ginseng Hairy Roots
    Zhang, Guofeng
    Zhou, Shengxue
    Zhao, Hai
    Xi, Guangsheng
    Gao, Yugang
    Wang, Yunjia
    INTERNATIONAL JOURNAL OF PHARMACOLOGY, 2018, 14 (05) : 615 - 623
  • [44] Characteristics of transformed Panax ginseng CA!Meyer hairy roots:: growth and nutrient profile
    Jeong, GT
    Park, DH
    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2006, 11 (01) : 43 - 47
  • [45] Effects of inoculum conditions on growth of hairy roots of Panax ginseng C.A. Meyer
    Jeong, GT
    Park, DH
    Ryu, HW
    Hwang, B
    Woo, JC
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2004, 113 (1-3) : 1193 - 1203
  • [46] Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages
    Lee, Jae Won
    Ji, Seung-Heon
    Lee, Young-Seob
    Choi, Doo Jin
    Choi, Bo-Ram
    Kim, Geum-Soog
    Baek, Nam-In
    Lee, Dae Young
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (06)
  • [47] Chromatographic analysis of ginsenosides occurring in the roots of American ginseng (Panax quinquefolium L.) and in Asian ginseng (Panax ginseng CA Mayer) preparations
    Ludwiczuk, A
    Wolski, T
    Berbec, S
    JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2002, 15 (02) : 147 - 150
  • [48] Effects of inoculum conditions on growth of hairy roots of Panax ginseng C. A. Meyer
    Gwi-Taek Jeong
    Don-Hee Park
    Hwa-Won Ryu
    Baik Hwang
    Je-Chang Woo
    Applied Biochemistry and Biotechnology, 2004, 116 : 1193 - 1203
  • [49] Antisense Suppression of Cycloartenol Synthase Results in Elevated Ginsenoside Levels in Panax ginseng Hairy Roots
    Liang, Yanlong
    Zhao, Shoujing
    Zhang, Xin
    PLANT MOLECULAR BIOLOGY REPORTER, 2009, 27 (03) : 298 - 304
  • [50] Comparative analysis of the essential oils from normal and hairy roots of Panax japonicas CA Meyer
    Zhang, Lai
    Zhang, Xian-Qiang
    Sun, Min
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (13): : 2440 - 2445