Ginsenosides in hairy roots of a Panax hybrid

被引：20
|
作者：
Washida, D
Shimomura, K
Nakajima, Y
Takido, M
Kitanaka, S
机构：
[1] Nihon Univ, Coll Pharm, Funabashi, Chiba 274, Japan
[2] Natl Inst Hlth Sci, Tsukuba Med Plant Res Stn, Tsukuba, Ibaraki 305, Japan
[3] Nagano Agr Technique & Training Ctr, Nagano 384, Japan
来源：
PHYTOCHEMISTRY | 1998年 / 49卷 / 08期
关键词：
Panax ginseng; Panax quinquefolium; Araliaceae; interspecific hybrid; hairy root culture; ginsenoside;
D O I：
10.1016/S0031-9422(98)00308-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hairy roots of an interspecific hybrid ginseng (Panax ginseng x P. quinquefolium), named Pgq, were established by the infection of Agrobacterium rhizogenes ATCC 15834. Growth and ginsenosides content of hairy roots cultured in various basal liquid media were measured periodically from 2 to 8 weeks. In Gamborg B5 liquid medium, the hairy roots showed best growth (5.87 g fresh weight per flask) at week 8. The highest content of ginsenoside was 2.87% as dry weight at week 8 when cultured in 1/8 Murashige-Skoog liquid medium. The ginsenoside content of Pgq hairy roots was comparable to that of Pgq root cultivated in the field. However the highest yield of ginsenosides was obtained in B5 liquid medium (3.85 mg per flask at week 8). (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：2331 / 2335
页数：5
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