Production of human erythropoietin by chimeric chickens

被引：38

作者：

Kodama, Daisuke ^{[1
]}

Nishimiya, Daisuke ^{[1
]}

Iwata, Ken-Ichi ^{[1
]}

Yamaguchi, Kazuhisa ^{[1
]}

Yoshida, Kazuhiro ^{[1
]}

Kawabe, Yoshinori ^{[1
]}

Motono, Makoto ^{[1
]}

Watanabe, Hiroyuki ^{[2
]}

Yamashita, Takashi ^{[2
]}

Nishijima, Ken-ichi ^{[1
]}

Kamihira, Masamichi ^{[1
]}

Iijima, Shinji ^{[1
]}

机构：

[1] Nagoya Univ, Dept Biotechnol, Chikusa Ku, Nagoya, Aichi 4648603, Japan

[2] Kaneka Corp, Takasago, Hyogo 6768688, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 367卷 / 04期

关键词：

transgenic chicken; retroviral vector; erythropoietin; carbohydrate;

D O I：

10.1016/j.bbrc.2008.01.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：834 / 839

页数：6

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