Production of human erythropoietin by chimeric chickens

被引：38
|
作者：
Kodama, Daisuke [1 ]
Nishimiya, Daisuke [1 ]
Iwata, Ken-Ichi [1 ]
Yamaguchi, Kazuhisa [1 ]
Yoshida, Kazuhiro [1 ]
Kawabe, Yoshinori [1 ]
Motono, Makoto [1 ]
Watanabe, Hiroyuki [2 ]
Yamashita, Takashi [2 ]
Nishijima, Ken-ichi [1 ]
Kamihira, Masamichi [1 ]
Iijima, Shinji [1 ]
机构：
[1] Nagoya Univ, Dept Biotechnol, Chikusa Ku, Nagoya, Aichi 4648603, Japan
[2] Kaneka Corp, Takasago, Hyogo 6768688, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 367卷 / 04期
关键词：
transgenic chicken; retroviral vector; erythropoietin; carbohydrate;
D O I：
10.1016/j.bbrc.2008.01.020
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：834 / 839
页数：6
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