Generation of SCN1A Knock out induced pluripotent stem cell (iPSC) line

被引：4

作者：

Shan, Wei ^{[1
,2
]}

Yang, Xiaoling ^{[3
]}

Ren, Qian ^{[4
,5
,6
]}

Wang, Qun ^{[1
,2
]}

机构：

[1] Capital Med Univ, Dept Neurol, Beijing Tiantan Hosp, Beijing 100070, Peoples R China

[2] Natl Ctr Clin Med Neurol Dis, Beijing 10070, Peoples R China

[3] Peking Univ First Hosp, Dept Pediat, 1 Xian Men St, Beijing 100034, Peoples R China

[4] Hebei Med Univ, Dept Human Anat, Shijiazhuang 050017, Hebei, Peoples R China

[5] Hebei Med Univ, Inst Med & Hlth Sci, Int Cooperat Lab Stem Cell Res, Shijiazhuang 050017, Hebei, Peoples R China

[6] Res Ctr Stem Cell Med Translat Engn, Shijiazhuang 050017, Hebei, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 55卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2021.102452

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The SCN1A gene encodes the voltage-gated Na+ channel alpha subunit Nav1.1 and is the most clinically relevant epilepsy gene. Variants in SCN1A result in a broad phenotypic spectrum of epilepsy syndromes, from mild genetic epilepsy with febrile seizures plus to severe Dravet syndrome (DS). Here, we generated a SCN1A-knockout human iPSC line via CRISPR/Cas9 gene editing. The resulting iPSCs had a normal karyotype, were free of genomically integrated epitomal plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential.

引用

页数：4

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