Synthesis and NMR analysis of 13C and 15N-labeled long-chain polyamines (LCPAs)

被引：4

作者：

Abacilar, Maryna ^{[1
]}

Daus, Fabian ^{[1
]}

Haas, Christian ^{[1
]}

Brueckner, Stephan Ingmar ^{[2
]}

Brunner, Eike ^{[2
]}

Geyer, Armin ^{[1
]}

机构：

[1] Univ Marburg, Fachbereich Chem, Hans Meerwein Str 4, D-35032 Marburg, Germany

[2] Tech Univ Dresden, Fachrichtung Chem & Lebensmittelchem, D-01062 Dresden, Germany

来源：

RSC ADVANCES | 2016年 / 6卷 / 96期

关键词：

SILICA NANOSPHERES; DIATOM BIOSILICA; SPECTROSCOPY; REDUCTION; RESONANCE; CLEAVAGE; MILD;

D O I：

10.1039/c6ra19624a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

N-15, C-13, or both isotope labels were introduced in selected positions of several LCPAs containing 5, 11, or 13 nitrogens. The number of transformations during chemical synthesis wasminimized by coupling Fmoc-glycine-N-15, Fmoc-glycine-1-C-13, or Fmoc-beta-alanine simultaneously at both ends of the growing oligoamide chain on resin-linked norspermidine. LCPAs containing 10-20 nitrogens are the main organic constituent of diatom biosilica. Preliminary NMR studies of bioinspired silica nanocomposites obtained from double-labeled LCPA 14 and Si-29-enriched orthosilicic acid identified the close spatial arrangement of Si-29 and C-13 nuclei.

引用

页码：93343 / 93348

页数：6

共 50 条

[21] A novel NMR experiment for the sequential assignment of proline residues and proline stretches in 13C/15N-labeled proteins
M.J. Bottomley
M.J. Macias
Z. Liu
M. Sattler
Journal of Biomolecular NMR, 1999, 13 : 381 - 385
[22] High-Resolution Studies of Uniformly 13C,15N-Labeled RNA by Solid-State NMR Spectroscopy
Cherepanov, Alexey V.
Glaubitz, Clemens
Schwalbe, Harald
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (28) : 4747 - 4750
[23] An optimized PCP-based procedure for production of 13C/15N-labeled DNA
Yan, JL
Bushweller, JH
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 284 (02) : 295 - 300
[24] 13C/15N distance determination by CPMAS NMR in uniformly 13C labeled molecules
Hologne, M
Raya, J
Hirschinger, J
MAGNETIC RESONANCE IN CHEMISTRY, 2006, 44 (02) : 174 - 177
[25] Efficient synthesis of 13C,15N-labeled RNA containing the cap structure m7GpppA
1600, ACS, Washington, DC, USA (122):
[26] Efficient synthesis of 13C,15N-labeled RNA containing the cap structure m7GpppA
Matsuo, H
Moriguchi, T
Takagi, T
Kusakabe, T
Buratowski, S
Sekine, M
Kyogoku, Y
Wagner, G
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (11) : 2417 - 2421
[27] A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins
Xu, YQ
Lin, Z
Ho, C
Yang, DW
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (34) : 11920 - 11921
[28] Chemical synthesis of 13C and 15N labeled nucleosides
Lagoja, IM
Herdewijn, P
SYNTHESIS-STUTTGART, 2002, (03): : 301 - 314
[29] Synthesis of 15N and 13C selectively labeled anandamide
Xie, XQ
Yang, HQ
Choi, G
Lin, SY
Makriyannis, A
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 2002, 45 (09): : 775 - 784
[30] Evaluation of the 13C NMR signals of saturated carbons in some long-chain compounds
Knothe, G
Nelsen, TC
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1998, (09): : 2019 - 2026

← 1 2 3 4 5 →