Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells

被引：0
|
作者：
Zhang, L [1 ]
Rui, YC [1 ]
Qiu, Y [1 ]
Li, TJ [1 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Pharmacol, Shanghai 200433, Peoples R China
来源：
ACTA PHARMACOLOGICA SINICA | 2001年 / 22卷 / 04期
关键词：
lysophosphatidylcholines; vascular endotheliun; growth substances; enzyme-linked immunosorbent assay; immunohistochemistry;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304). METHODS: VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization. RESULTS: Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time-and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells. CONCLUSION: LPC could induce a strong expression of VEGF in ECV304 cells.
引用
下载
收藏
页码：335 / 339
页数：5
相关论文
共 50 条
  • [41] Apoptosis of endothelial cell line ECV304 persistently infected with Orientia tsutsugamushi
    Kim, MK
    Kee, SH
    Cho, KA
    Chung, MH
    Lim, BU
    Chang, WH
    Kang, JS
    MICROBIOLOGY AND IMMUNOLOGY, 1999, 43 (08) : 751 - 757
  • [42] Characterisation of the P2-receptor(s) located on ECV304 endothelial cells using FLIPR®.
    Law, CE
    Lydford, SJ
    Henderson, DJ
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1998, 358 (01) : R126 - R126
  • [43] Modulation of mitochondrial Ca2+ in ECV304 endothelial cells by agents which elevate cAMP
    Lawrie, AM
    Zolle, O
    Simpson, AWM
    CELL CALCIUM, 1997, 22 (04) : 229 - 234
  • [44] Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A
    Hong, Susie
    Chang, Seo-Yoon
    Yeom, Dong-Hoon
    Kang, Jung-Hoon
    Hong, Kyong-Ja
    ANTI-CANCER DRUGS, 2007, 18 (09) : 1005 - 1014
  • [45] Modulation of ICAM-1 expression in ECV304 cells by macrophage-released cytokines
    Antonelli, A
    Bianchi, M
    Crinelli, R
    Gentilini, L
    Magnani, M
    BLOOD CELLS MOLECULES AND DISEASES, 2001, 27 (06) : 978 - 991
  • [46] Effect of Lipopolysaccharide Mediating Early- and Late- Activated THP-1 Macrophages on ECV304 Endothelial Cell Dysfunction: Dysregulation of Secretion of VEGF and Proliferation and Migration of ECV304
    Chu, Fu Jiang
    Jin, Xiao Bao
    Xu, Yin Ye
    Ma, Yan
    Li, Xiao Bo
    Lu, Xue Mei
    Liu, Wen Bin
    Zhu, Jia Yong
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2013, 31 (2-3) : 400 - 407
  • [47] LYSOPHOSPHATIDYLCHOLINE INDUCES GROWTH-FACTOR GENE-EXPRESSION IN CULTURED HUMAN VASCULAR ENDOTHELIAL-CELLS
    KUME, N
    GIMBRONE, MA
    CIRCULATION, 1993, 88 (04) : 228 - 228
  • [48] Induction of tissue factor expression and release as microparticles in ECV304 cell line by Chlamydia pneumoniae infection
    Ettelaie, Camille
    Collier, Mary E. W.
    James, Nicola J.
    Li, Chao
    ATHEROSCLEROSIS, 2007, 190 (02) : 343 - 351
  • [49] Effect of troglitazone on vascular endothelial growth factor expression in vascular smooth muscle cells
    Yamakawa, K
    Hosoi, M
    Koyama, H
    Tanaka, S
    Emoto, M
    Kawagishi, T
    Shioi, A
    Okuno, Y
    Morii, H
    Nishizawa, Y
    CIRCULATION, 1999, 100 (18) : 40 - 40
  • [50] Staurosporine induces apoptotic volume decrease (AVD) in ECV304 cells
    Porcelli, AM
    Ghelli, A
    Zanna, C
    Valente, P
    Ferroni, S
    Rugolo, M
    APOPTOSIS: FROM SIGNALING PATHWAYS TO THERAPEUTIC TOOLS, 2003, 1010 : 342 - 346
12345