Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells

被引：0
|
作者：
Zhang, L [1 ]
Rui, YC [1 ]
Qiu, Y [1 ]
Li, TJ [1 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Pharmacol, Shanghai 200433, Peoples R China
来源：
ACTA PHARMACOLOGICA SINICA | 2001年 / 22卷 / 04期
关键词：
lysophosphatidylcholines; vascular endotheliun; growth substances; enzyme-linked immunosorbent assay; immunohistochemistry;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304). METHODS: VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization. RESULTS: Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time-and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells. CONCLUSION: LPC could induce a strong expression of VEGF in ECV304 cells.
引用
下载
收藏
页码：335 / 339
页数：5
相关论文
共 50 条
  • [21] Expression of cadherin-5 in ECV304 cells increases barrier function
    Radel, CG
    Russ, PK
    Glick, GG
    Haselton, FR
    Davidson, MK
    FASEB JOURNAL, 1999, 13 (04): : A529 - A529
  • [22] Regulation of Ca2+ movements by cyclovirobuxine D in ECV304 endothelial cells
    Grossini, E
    Avanzi, G
    Gallicchio, M
    Molinari, C
    Vacca, G
    Bellomo, G
    PHARMACOLOGICAL RESEARCH, 2005, 52 (02) : 154 - 161
  • [23] Protective effect of quercetin on the homocysteine-injured human umbilical vein vascular endothelial cell line (ECV304)
    Lin, Rong
    Liu, Juntian
    Gan, Weijie
    Ding, Cunjing
    BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, 2007, 101 (03) : 197 - 202
  • [24] Influence of the anticancer drug busulfan on activin A expression in ECV304
    Dressel, D
    Sperker, B
    Ritter, CA
    Kroemer, HK
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2002, 365 : R119 - R119
  • [25] Ultrasound/Microbubble Enhances Foreign Gene Expression in ECV304 Cells and Murine Myocardium
    Dongping GUO
    1 Cardiovascular Division
    Acta Biochimica et Biophysica Sinica, 2004, (12) : 824 - 831
  • [26] Identification of adenosine receptor subtypes expressed in the human endothelial-like ECV304 cells
    Kobayashi, R
    Saitoh, O
    Nakata, H
    PHARMACOLOGY, 2005, 74 (03) : 143 - 151
  • [27] Characterization of G protein-coupled receptors expressed by ECV304 human endothelial cells
    Howl, J
    Mondszein, RH
    Wheatley, M
    ENDOTHELIUM-JOURNAL OF ENDOTHELIAL CELL RESEARCH, 1998, 6 (01): : 23 - 32
  • [28] HUMAN ENDOTHELIAL-CELL LINE, ECV304, PRODUCES PROUROKINASE
    TAKAHASHI, K
    SAWASAKI, Y
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1991, 27 (10): : 766 - 768
  • [29] Ultrasound/microbubble enhances foreign gene expression in ECV304 cells and murine myocardium
    Guo, DP
    Li, XY
    Sun, P
    Wang, ZG
    Chen, XY
    Chen, Q
    Fan, LM
    Zhang, B
    Shao, LZ
    Li, XR
    ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2004, 36 (12) : 824 - 831
  • [30] Effect of Porphyromonas gingivalis PrtC on cytokine expression in ECV304 endothelial cells and its level in subgingival plaques from patients with chronic periodontitis
    Wu, Yan-min
    Chen, Li-li
    Yan, Jie
    Zhuang, Chun-yan
    Gu, Zhi-yuan
    ACTA PHARMACOLOGICA SINICA, 2007, 28 (07) : 1015 - 1023
12345