Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells

被引：0
|
作者：
Zhang, L [1 ]
Rui, YC [1 ]
Qiu, Y [1 ]
Li, TJ [1 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Pharmacol, Shanghai 200433, Peoples R China
来源：
ACTA PHARMACOLOGICA SINICA | 2001年 / 22卷 / 04期
关键词：
lysophosphatidylcholines; vascular endotheliun; growth substances; enzyme-linked immunosorbent assay; immunohistochemistry;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304). METHODS: VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization. RESULTS: Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time-and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells. CONCLUSION: LPC could induce a strong expression of VEGF in ECV304 cells.
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页码：335 / 339
页数：5
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