Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes

被引：5
|
作者：
Filatov, M. A. [1 ]
Nikishin, D. A. [1 ,2 ]
Khramova, Y., V [1 ]
Semenova, M. L. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Fac Biol, Leninskie Gory 1,Bid 12, Moscow 119991, Russia
[2] Russian Acad Sci, NK Koltzov Inst Dev Biol, Vaviiova St 26, Moscow 119334, Russia
来源：
ZYGOTE | 2019年 / 27卷 / 06期
基金：
俄罗斯科学基金会;
关键词：
Germinal vesicle; Mice; Oocyte; PCR; Reference gene; BETA-ACTIN; EXPRESSION; IDENTIFICATION; RNA;
D O I：
10.1017/S0967199419000492
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.
引用
收藏
页码：392 / 397
页数：6
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