Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes

被引：5

作者：

Filatov, M. A. ^{[1
]}

Nikishin, D. A. ^{[1
,2
]}

Khramova, Y., V ^{[1
]}

Semenova, M. L. ^{[1
]}

机构：

[1] Lomonosov Moscow State Univ, Fac Biol, Leninskie Gory 1,Bid 12, Moscow 119991, Russia

[2] Russian Acad Sci, NK Koltzov Inst Dev Biol, Vaviiova St 26, Moscow 119334, Russia

来源：

ZYGOTE | 2019年 / 27卷 / 06期

基金：

俄罗斯科学基金会;

关键词：

Germinal vesicle; Mice; Oocyte; PCR; Reference gene; BETA-ACTIN; EXPRESSION; IDENTIFICATION; RNA;

D O I：

10.1017/S0967199419000492

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.

引用

页码：392 / 397

页数：6

共 50 条

[21] Quantitative Evaluation of Reference Genes for Real-Time PCR During In Vitro Maturation of Ovine Oocytes
O'Connor, T.
Wilmut, I.
Taylor, J.
REPRODUCTION IN DOMESTIC ANIMALS, 2013, 48 (03) : 477 - 483
[22] Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba
Huang, Tian
Long, Jianmei
Liu, Si-Wen
Yang, Zi-Wei
Zhu, Qi-Jin
Zhao, Xiao-Lan
Peng, Changcao
SCIENTIFIC REPORTS, 2018, 8
[23] Selection and validation of reference genes for quantitative real-time PCR analysis of gene expression in Cichorium intybus
Delporte, Marianne
Legrand, Guillaume
Hilbert, Jean-Louis
Gagneul, David
FRONTIERS IN PLANT SCIENCE, 2015, 6
[24] Selection and evaluation of reference genes for quantitative real-time PCR analysis in lac insect (Kerria lacca)
Wang, Weiwei
Ling, Xiaofei
Bashir, Nawaz Haider
Lu, Qin
Zhang, Jinwen
Li, Tichu
Chen, Hang
ENTOMOLOGICAL RESEARCH, 2022, 52 (02) : 57 - 67
[25] Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba
Tian Huang
Jianmei Long
Si-Wen Liu
Zi-Wei Yang
Qi-Jin Zhu
Xiao-Lan Zhao
Changcao Peng
Scientific Reports, 8
[26] Selection of reference genes for quantitative real-time PCR normalization in European quail tissues
de Sousa, Fabiana Cristina Belchior
do Nascimento, Carlos Souza
Macario, Maise dos Santos
Araujo, Renan dos Santos
Barbosa, Leandro Teixeira
Bayao, Geraldo Fabio Viana
Sousa, Katiene Regia Silva
MOLECULAR BIOLOGY REPORTS, 2021, 48 (01) : 67 - 76
[27] Selection of stable reference genes for quantitative real-time PCR in porcine gastrointestinal tissues
Ryan, M. T.
Collins, C. B.
O'Doherty, J. V.
Sweeney, T.
LIVESTOCK SCIENCE, 2010, 133 (1-3) : 42 - 44
[28] Selection of reference genes for quantitative real-time PCR normalization in European quail tissues
Fabiana Cristina Belchior de Sousa
Carlos Souza do Nascimento
Maíse dos Santos Macário
Renan dos Santos Araújo
Leandro Teixeira Barbosa
Geraldo Fábio Viana Bayão
Katiene Régia Silva Sousa
Molecular Biology Reports, 2021, 48 : 67 - 76
[29] Selection of reliable reference genes for quantitative real-time RT-PCR in alfalfa
Wang, Xuemin
Fu, Yuanyuan
Ban, Liping
Wang, Zan
Feng, Guangyan
Li, Jun
Gao, Hongwen
GENES & GENETIC SYSTEMS, 2015, 90 (03) : 175 - 180
[30] Reference gene expression stability and selection for quantitative real-time PCR in mouse myocardium
Everaert, B.
Timmermans, J. P.
Vrints, C.
EUROPEAN HEART JOURNAL, 2010, 31 : 536 - 536

← 1 2 3 4 5 →