Crystallization and preliminary X-ray diffraction studies of DNA decamer d(CCAGGCCTGG) complexed with cobalt(III)-pepleomycin

被引:0
|
作者
Yaguma, K
Matsumoto, O
Guan, LL
Kawano, Y
Kamiya, N
Tsujimoto, G
Sugiura, Y
机构
[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Genom Drug Discovery, Sakyo Ku, Kyoto 6068501, Japan
[2] SPring8, RIKEN Harima Inst, Mikazuki, Hyogo 6795148, Japan
[3] Marine Biotechnol Inst, Shimizu Labs, Shimizu, Shizuoka 4240037, Japan
[4] Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan
关键词
D O I
10.1107/S0907444903021917
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Crystals of the self-complementary oligonucleotide d(CCAGGCCTGG) [Heinemann & Alings ( 1989), J. Mol. Biol. 210, 369 - 381] complexed with the hydroperoxide of cobalt-pepleomycin (CoPEP) were obtained by the hanging-drop vapour-diffusion method at 298 K. An X-ray diffraction data set was collected to 2.8 Angstrom at 100 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.52, b = 59.88, c = 72.93 Angstrom.
引用
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页码:144 / 145
页数:2
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