Crystallization and preliminary X-ray diffraction studies of mammalian purple acid phosphatase

被引：3
|
作者：
Guddat, LW [1 ]
McAlpine, AS
Hume, D
de Jersey, J
Hamilton, S
Martin, JL
机构：
[1] Univ Queensland, Dept Biochem, St Lucia, Qld 4072, Australia
[2] Univ Queensland, Ctr Drug Design & Dev, St Lucia, Qld 4072, Australia
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444999006241
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The oxidized form of purple acid phosphatase from pig allantoic fluid has been crystallized in the presence of phosphate using the hanging-drop technique. The crystals belong to the space group P2(1)2(1)2(1) and have unit-cell parameters a = 66.8, b = 70.3, c = 78.7 Angstrom. Diffraction data collected from a cryocooled crystal using a conventional X-ray source extend to 1.55 Angstrom resolution. A knowledge of the three-dimensional structure of mammalian purple acid phosphatase will aid in understanding the substrate specificity of the enzyme and will be important in the rational design of inhibitors, with potential in the treatment of bone diseases.
引用
收藏
页码：1462 / 1464
页数：3
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