Amperometric glucose microbiosensor based on a Prussian Blue modified carbon fiber electrode for physiological applications

Results of experiments in vitro and in vivo, using an amperometric glucose microbiosensor based on a Prussian Blue (PB) modified carbon fiber electrode with very low dimensions (similar to 10 mu m diameter), are presented. The electrocatalytic properties of the PB film enable detection of an enzymatic by-product (H2O2) at a very low applied potential: 0.0 V against SCE. The main steps during glucose microbiosensor construction were examined by cyclic voltammetry and electrochemical impedance spectroscopy. Excellent selectivity of the glucose microbiosensors against a large number of physiological interference compounds is demonstrated. Finally, microbiosensor responses during intraperitoneal injection, local infusion and local electrical stimulation showed sufficient sensitivity and stability to monitor multiphasic and reversible changes in brain ECF glucose levels during physiological experiments, illustrating the excellent properties and utility of this biosensor design in the neurosciences. (C) 2010 Elsevier B.V. All rights reserved.