Extractive spectrophotometric determination of fluconazale by ion-pair cornplex formation with bromocresol green

An extraction-spectrophotometric method for the determination of trace amounts of fluconazole was described. Fluconazole was effectively extracted as a I : I ion-pair complex with bromocresole green (BCG) at pH 3.0 into chloroform, followed by spectrophotometric determination at 420 run. Beer's law was obeyed over the range of 450 mu g center dot mL(-1) of fluconazole with a detection limit of 3.7 mu g center dot mL(-1). The method is simple, rapid and sensitive. The procedure was applied to the determination of fluconazole in pharmaceutical preparations as well as its recovery from a blood serum sample.