Δ6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis

被引：8

作者：

Liu, Jianmin ^{[1
,2
]}

Li, Dianrong ^{[1
,3
]}

Yin, Yongtai ^{[1
]}

Wang, Hao ^{[3
]}

Li, Maoteng ^{[1
]}

Yu, Longjiang ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China

[2] Henan Polytech Univ, Dept Biotechnol, Inst Resources & Environm, Jiaozuo 454003, Peoples R China

[3] Hybrid Rapeseed Res Ctr Shaanxi Prov, Dali 715105, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 10期

基金：

国家高技术研究发展计划(863计划);

关键词：

Cloning and expression; Delta(6)-fatty acid desaturase; gamma-Linolenic acid; Mortierella alpina; Phylogenetic analysis; GAMMA-LINOLENIC ACID; ARACHIDONIC-ACID; HETEROLOGOUS EXPRESSION; MUCOR-ROUXII; DESATURASE; GENE;

D O I：

10.1007/s10529-011-0650-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The open reading frame of the Delta(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Delta(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of linoleic acid to gamma-linolenic acid but not the conversion of alpha-linolenic acid to octadecatetraenoic acid. The results indicate that the Delta(6)-desaturase gene from M. alpina W15 has substrate specificity in different organisms. Phylogenetic analysis revealed that Delta(6)-desaturase genes can be divided into four monophyletic groups. This work paves the way for further study of the functions of Delta(6)-desaturase in fatty acid metabolism and its three-dimensional structure.

引用

页码：1985 / 1991

页数：7

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