Δ6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis

被引：8
|
作者：
Liu, Jianmin [1 ,2 ]
Li, Dianrong [1 ,3 ]
Yin, Yongtai [1 ]
Wang, Hao [3 ]
Li, Maoteng [1 ]
Yu, Longjiang [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China
[2] Henan Polytech Univ, Dept Biotechnol, Inst Resources & Environm, Jiaozuo 454003, Peoples R China
[3] Hybrid Rapeseed Res Ctr Shaanxi Prov, Dali 715105, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 10期
基金：
国家高技术研究发展计划(863计划);
关键词：
Cloning and expression; Delta(6)-fatty acid desaturase; gamma-Linolenic acid; Mortierella alpina; Phylogenetic analysis; GAMMA-LINOLENIC ACID; ARACHIDONIC-ACID; HETEROLOGOUS EXPRESSION; MUCOR-ROUXII; DESATURASE; GENE;
D O I：
10.1007/s10529-011-0650-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The open reading frame of the Delta(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Delta(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of linoleic acid to gamma-linolenic acid but not the conversion of alpha-linolenic acid to octadecatetraenoic acid. The results indicate that the Delta(6)-desaturase gene from M. alpina W15 has substrate specificity in different organisms. Phylogenetic analysis revealed that Delta(6)-desaturase genes can be divided into four monophyletic groups. This work paves the way for further study of the functions of Delta(6)-desaturase in fatty acid metabolism and its three-dimensional structure.
引用
收藏
页码：1985 / 1991
页数：7
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