Scanning for nucleotide variations in mitochondrial DNA fragments of Schistosoma japonicum by single-strand conformation polymorphism

被引：37
|
作者：
Bogh, HO
Zhu, XQ
Qian, BZ
Gasser, RB
机构：
[1] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia
[2] Danish Ctr Expt Parasitol, DK-1870 Frederiksberg C, Denmark
[3] Zhejiang Acad Med Sci, Inst Parasit Dis, WHO, Collaborating Ctr Helminthiasis, Hangzhou, Peoples R China
来源：
PARASITOLOGY | 1999年 / 118卷
关键词：
Schistosoma japonicum; mitochondrial DNA; NADH dehydrogenase 1; cytochrome c oxidase subunit I; mutations;
D O I：
10.1017/S0031182098003527
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
In this study, we employed a mutation scanning approach for the direct visual display of genetic variability in mitochondrial DNA (mtDNA) fragments within and among populations of Schistosoma japonicum from the People's Republic of China. Fragments of the NADH dehydrogenase 1 gene (ND1) and the cytochrome c oxidase subunit I (COI) were individually amplified front parasite DNA by polymerase chain reaction (PCR), denatured and subjected to single-strand conformation polymorphism (SSCP) analysis. Using ND1 and COI fragments, individuals representing different genotypes could be readily identified based on characteristic and reproducible SSCP profiles. The results demonstrated the usefulness of SSCP for the direct visual display of low-level sequence variation in mtDNA of S. japonicum prior to DNA sequence analysis. This approach has important implications for studying the genetic structure and biology of S. japonicum populations, and for analysing the inheritance of mitochondrial DNA.
引用
收藏
页码：73 / 82
页数：10
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