Development of an integrated in situ analyzer for quantitative analysis of microbial ATP in aquatic environments

被引：0
|
作者：
Fukuba, T. [1 ]
Fukuzawa, N.
Glutz, L. S.
Mlyaji, A.
Fujii, T.
机构：
[1] Univ Tokyo, Inst Ind Sci, Tokyo, Japan
[2] Ecole Polytech Fed Lausanne, Lausanne, Switzerland
来源：
2007 SYMPOSIUM ON UNDERWATER TECHNOLOGY AND WORKSHOP ON SCIENTIFIC USE OF SUBMARINE CABLES AND RELATED TECHNOLOGIES, VOLS 1 AND 2 | 2007年
关键词：
D O I：
10.1109/UT.2007.370802
中图分类号：
TP24 [机器人技术];
学科分类号：
080202 ; 1405 ;
摘要：
An integrated and miniaturized device for in situ quantitative analysis of microbial ATP was developed in this study. The device can perform extracellular ATP elimination, cell lysis and ATP quantification in a microfluidic chip. A pattern of a microchannel has been optimized to measure luminescence caused by luciferin-luciferase reaction. As a result of evaluation of the developed microfluidic device, 10 nM to 100 square M of ATP was successfully quantified. Environmental samples and bacterial culture media were also analyzed by the microfluidic device. Towards in situ calibration of the ATP quantitative analysis device, a prototype of caged ATP based online calibration component was also fabricated and evaluated.
引用
收藏
页码：240 / 244
页数：5
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