Development of an integrated in situ analyzer for quantitative analysis of microbial ATP in aquatic environments

被引：0

作者：

Fukuba, T. ^{[1
]}

Fukuzawa, N.

Glutz, L. S.

Mlyaji, A.

Fujii, T.

机构：

[1] Univ Tokyo, Inst Ind Sci, Tokyo, Japan

[2] Ecole Polytech Fed Lausanne, Lausanne, Switzerland

来源：

2007 SYMPOSIUM ON UNDERWATER TECHNOLOGY AND WORKSHOP ON SCIENTIFIC USE OF SUBMARINE CABLES AND RELATED TECHNOLOGIES, VOLS 1 AND 2 | 2007年

关键词：

D O I：

10.1109/UT.2007.370802

中图分类号：

TP24 [机器人技术];

学科分类号：

080202 ; 1405 ;

摘要：

An integrated and miniaturized device for in situ quantitative analysis of microbial ATP was developed in this study. The device can perform extracellular ATP elimination, cell lysis and ATP quantification in a microfluidic chip. A pattern of a microchannel has been optimized to measure luminescence caused by luciferin-luciferase reaction. As a result of evaluation of the developed microfluidic device, 10 nM to 100 square M of ATP was successfully quantified. Environmental samples and bacterial culture media were also analyzed by the microfluidic device. Towards in situ calibration of the ATP quantitative analysis device, a prototype of caged ATP based online calibration component was also fabricated and evaluated.

引用

页码：240 / 244

页数：5

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