Development of an integrated in situ analyzer for quantitative analysis of microbial ATP in aquatic environments

被引：0
|
作者：
Fukuba, T. [1 ]
Fukuzawa, N.
Glutz, L. S.
Mlyaji, A.
Fujii, T.
机构：
[1] Univ Tokyo, Inst Ind Sci, Tokyo, Japan
[2] Ecole Polytech Fed Lausanne, Lausanne, Switzerland
来源：
2007 SYMPOSIUM ON UNDERWATER TECHNOLOGY AND WORKSHOP ON SCIENTIFIC USE OF SUBMARINE CABLES AND RELATED TECHNOLOGIES, VOLS 1 AND 2 | 2007年
关键词：
D O I：
10.1109/UT.2007.370802
中图分类号：
TP24 [机器人技术];
学科分类号：
080202 ; 1405 ;
摘要：
An integrated and miniaturized device for in situ quantitative analysis of microbial ATP was developed in this study. The device can perform extracellular ATP elimination, cell lysis and ATP quantification in a microfluidic chip. A pattern of a microchannel has been optimized to measure luminescence caused by luciferin-luciferase reaction. As a result of evaluation of the developed microfluidic device, 10 nM to 100 square M of ATP was successfully quantified. Environmental samples and bacterial culture media were also analyzed by the microfluidic device. Towards in situ calibration of the ATP quantitative analysis device, a prototype of caged ATP based online calibration component was also fabricated and evaluated.
引用
收藏
页码：240 / 244
页数：5
相关论文
共 50 条
  • [1] Development of in situ microbial ATP analyzer and internal standard calibration method
    Hanatani, Kohei
    Fujii, Teruo
    Fukuba, Tatsuhiro
    2015 IEEE UNDERWATER TECHNOLOGY (UT), 2015,
  • [2] A microfluidic in situ analyzer for ATP quantification in ocean environments
    Fukuba, Tatsuhiro
    Aoki, Yusuke
    Fukuzawa, Noriyuki
    Yamamoto, Takatoki
    Kyo, Masanori
    Fujii, Teruo
    LAB ON A CHIP, 2011, 11 (20) : 3508 - 3515
  • [3] Development of in situ ATP quantitative analysis system "IISA-ATP"
    Fukuzawa, Noriyuki
    Fukuba, Tatsuhiro
    Fujii, Teruo
    2006 INTERNATIONAL CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE AND BIOLOGY, 2006, : 260 - +
  • [4] Integrated in situ genetic analyzer for microbiology in extreme environments
    Fukuba, Tatsuhiro
    Miyaji, Akimitsu
    Okamoto, Takuji
    Yamamoto, Takatoki
    Kaneda, Shohei
    Fujii, Teruo
    RSC ADVANCES, 2011, 1 (08) : 1567 - 1573
  • [5] Measurement and interpretation of microbial adenosine tri-phosphate (ATP) in aquatic environments
    Hammes, Frederik
    Goldschmidt, Felix
    Vital, Marius
    Wang, Yingying
    Egli, Thomas
    WATER RESEARCH, 2010, 44 (13) : 3915 - 3923
  • [6] Development and evaluation of the integrated in situ analyzer for gene-"IISA-Gene" for microbiology in extreme environments
    Fukuba, Tatsuhiro
    Fujii, Teruo
    2006 INTERNATIONAL CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE AND BIOLOGY, 2006, : 184 - +
  • [7] Calibration of a passive, in situ, integrative sampler for monitoring of microbial biotoxins in aquatic environments
    Nyoni, Hlengilizwe
    Mamba, Bhekie B.
    Msagati, Titus A. M.
    WATER SCIENCE AND TECHNOLOGY-WATER SUPPLY, 2015, 15 (06): : 1353 - 1367
  • [8] Quantitative analysis of human enteric adenoviruses in aquatic environments
    Haramoto, E.
    Katayama, H.
    Oguma, K.
    Ohgaki, S.
    JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (06) : 2153 - 2159
  • [9] Quantitative analysis of nifH genes and transcripts from aquatic environments
    Short, SM
    Zehr, JP
    ENVIRONMENTAL MICROBIOLOGY, 2005, 397 : 380 - 394
  • [10] Development of a passive, in situ, integrative sampler for hydrophilic organic contaminants in aquatic environments
    Alvarez, DA
    Petty, JD
    Huckins, JN
    Jones-Lepp, TL
    Getting, DT
    Goddard, JP
    Manahan, SE
    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2004, 23 (07) : 1640 - 1648
12345