An ultrasensitive colorimetric method for the assay of endo-1,4-beta-D-glucanase

被引：2
|
作者：
Sestak, S [1 ]
Farkas, V [1 ]
机构：
[1] SLOVAK ACAD SCI,INST CHEM,BRATISLAVA 84238,SLOVAKIA
来源：
BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 10期
关键词：
D O I：
10.1007/BF00222556
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A colorimetric method for the assay of endo-1,4-beta-D-glucanase from fungal cellulolytic systems which utilizes xyloglucan as substrate is described. The substrate forms a blue-green complex with I-2 which is lost upon its breakdown with the enzyme. The new assay is a simple one-step procedure and its sensitivity is comparable with that of viscometry.
引用
收藏
页码：731 / 734
页数：4
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