An ultrasensitive colorimetric method for the assay of endo-1,4-beta-D-glucanase

被引：2

作者：

Sestak, S ^{[1
]}

Farkas, V ^{[1
]}

机构：

[1] SLOVAK ACAD SCI,INST CHEM,BRATISLAVA 84238,SLOVAKIA

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 10期

关键词：

D O I：

10.1007/BF00222556

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A colorimetric method for the assay of endo-1,4-beta-D-glucanase from fungal cellulolytic systems which utilizes xyloglucan as substrate is described. The substrate forms a blue-green complex with I-2 which is lost upon its breakdown with the enzyme. The new assay is a simple one-step procedure and its sensitivity is comparable with that of viscometry.

引用

页码：731 / 734

页数：4

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