user

Inhibition of the subgenomic hepatitis C virus replicon in Huh-7 cells by 2′-deoxy-2′-fluorocytidine

被引：61
|
作者：
Stuyver, LJ
McBrayer, TR
Whitaker, T
Tharnish, PM
Ramesh, M
Lostia, S
Cartee, L
Shi, JX
Hobbs, A
Schinazi, RF
Watanabe, KA
Otto, MJ
机构：
[1] Pharmasset Inc, Tucker, GA 30084 USA
[2] Emory Univ, Sch Med, Vet Affairs Med Ctr, Dept Pediat, Decatur, GA 30033 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 02期
关键词：
D O I：
10.1128/AAC.48.2.651-654.2004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
2'-Deoxy-2'-fluorocytidine (FdC) is a potent inhibitor of the hepatitis C virus RNA replicon in culture, and FdC-5'-triphosphate is an effective inhibitor of the NS5B polymerase. Dynamic profiling of cell growth in an antiviral assay showed that FdC caused cytostasis due to an S-phase arrest. These observations demonstrate that FdC treatment is affecting both a viral target and a cellular target.
引用
收藏
页码：651 / 654
页数：4
相关论文
共 50 条
  • [1] Mathematical modeling of subgenomic hepatitis C virus replication in Huh-7 cells
    Dahari, Harel
    Ribeiro, Ruy M.
    Rice, Charles M.
    Perelson, Alan S.
    JOURNAL OF VIROLOGY, 2007, 81 (02) : 750 - 760
  • [2] Dynamics of subgenomic hepatitis C virus replicon RNA levels in Huh-7 cells after exposure to nucleoside antimetabolites
    Stuyver, LJ
    McBrayer, TR
    Tharnish, PM
    Hassan, AEA
    Chu, CK
    Pankiewicz, KW
    Watanabe, KA
    Schinazi, RF
    Otto, MJ
    JOURNAL OF VIROLOGY, 2003, 77 (19) : 10689 - 10694
  • [3] Inhibition of subgenomic hepatitis C virus RNA in Huh-7 cells: ribavirin induces mutagenesis in HCV RNA
    Kanda, T
    Yokosuka, O
    Imazeki, F
    Tanaka, M
    Shino, Y
    Shimada, H
    Tomonaga, T
    Nomura, F
    Nagao, K
    Ochiai, T
    Saisho, H
    JOURNAL OF VIRAL HEPATITIS, 2004, 11 (06) : 479 - 487
  • [4] Expression of the subgenomic hepatitis C virus replicon alters iron homeostasis in Huh7 cells
    Fillebeen, C.
    Muckenthaler, M.
    Andriopoulos, B.
    Bisaillon, M.
    Mounir, Z.
    Hentze, M. W.
    Koromilas, A. E.
    Pantopoulos, K.
    AMERICAN JOURNAL OF HEMATOLOGY, 2007, 82 (06) : 580 - 580
  • [5] Mathematical modeling of subgenomic hepatitis C viral replication in Huh-7 cells
    Dahari, H.
    Ribeiro, R. M.
    Rice, C. M.
    Perelson, A. S.
    JOURNAL OF HEPATOLOGY, 2006, 44 : S273 - S273
  • [6] Expression of the subgenomic hepatitis C virus replicon alters iron homeostasis in Huh7 cells
    Fillebeen, Carine
    Muckenthaler, Martina
    Andricipoulos, Bill
    Bisaillon, Martin
    Mounir, Zineb
    Hentze, Matthias W.
    Koromilas, Antonis E.
    Pantopoulos, Kostas
    JOURNAL OF HEPATOLOGY, 2007, 47 (01) : 12 - 22
  • [7] Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons
    Gosert, R
    Egger, D
    Lohmann, V
    Bartenschlager, R
    Blum, HE
    Bienz, K
    Moradpour, D
    JOURNAL OF VIROLOGY, 2003, 77 (09) : 5487 - 5492
  • [8] Hepatitis A protein VP1-2A reduced cell viability in Huh-7 cells with hepatitis C virus subgenomic RNA replication
    Kanda, T
    Yokosuka, O
    Imazeki, F
    Fujiwara, K
    Saisho, H
    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2006, 21 (03) : 625 - 626
  • [9] Hepatitis B virus and hepatitis C virus interaction in Huh-7 cells
    Eyre, Nicholas S.
    Phillips, Renee J.
    Bowden, Scott
    Yip, Evelyn
    Dewar, Ben
    Locarnini, Stephen A.
    Beard, Michael R.
    JOURNAL OF HEPATOLOGY, 2009, 51 (03) : 446 - 457
  • [10] INHIBITION OF CELL-GROWTH BY 2'-DEOXY-2'-FLUOROCYTIDINE
    BROX, LW
    LEPAGE, GA
    HENDLER, SS
    SHANNAHO.DH
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1974, 15 (MAR): : 26 - 26
12345