Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene

被引：14
|
作者：
Maezawa, Masaki [1 ,2 ]
Watanabe, Ken-ichi [2 ]
Horiuchi, Noriyuki [1 ,2 ]
Matsumoto, Kotaro [1 ,2 ]
Kobayashi, Yoshiyasu [1 ,2 ]
Inokuma, Hisashi [1 ,2 ]
机构：
[1] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan
[2] Obihiro Univ Agr & Vet Med, Dept Vet Med, Obihiro, Hokkaido 0808555, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 01期
关键词：
bovine B-cell lymphoma; clonality; polymerase chain reaction; PCR;
D O I：
10.1292/jvms.19-0418
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma.
引用
收藏
页码：61 / 63
页数：3
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