Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene

被引：14

作者：

Maezawa, Masaki ^{[1
,2
]}

Watanabe, Ken-ichi ^{[2
]}

Horiuchi, Noriyuki ^{[1
,2
]}

Matsumoto, Kotaro ^{[1
,2
]}

Kobayashi, Yoshiyasu ^{[1
,2
]}

Inokuma, Hisashi ^{[1
,2
]}

机构：

[1] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan

[2] Obihiro Univ Agr & Vet Med, Dept Vet Med, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 01期

关键词：

bovine B-cell lymphoma; clonality; polymerase chain reaction; PCR;

D O I：

10.1292/jvms.19-0418

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma.

引用

页码：61 / 63

页数：3

共 50 条

[21] ANALYSIS OF IMMUNOGLOBULIN HEAVY-CHAIN GENE REARRANGEMENT USING THE POLYMERASE CHAIN-REACTION - REPLY
TRAINOR, KJ
BRISCO, MJ
WAN, JH
NEOH, S
GRIST, S
MORLEY, AA
BLOOD, 1992, 79 (01) : 292 - 292
[22] IMMUNOGLOBULIN GENE REARRANGEMENT OF B-CELL NONHODGKINS LYMPHOMAS (B-NHL) BY POLYMERASE CHAIN-REACTION
SZABOLCS, MJ
YEE, HT
CESARMAN, E
INGHIRAMI, G
KNOWLES, DM
FASEB JOURNAL, 1992, 6 (05): : A1609 - A1609
[23] B-cell clonality analysis by polymerase chain reaction (PCR) detection of immunoglobulin (Ig) κ and λ light chain gene rearrangements.
Chiu, A
Diss, T
Knowles, DM
Pan, LX
MODERN PATHOLOGY, 2001, 14 (01) : 159A - 159A
[24] Immunoglobulin heavy chain gene analysis of ocular adnexal extranodal marginal zone B-cell lymphoma
Hara, Y
Nakamura, N
Kuze, T
Hashimoto, Y
Sasaki, Y
Shirakawa, A
Furuta, M
Yago, K
Kato, K
Abe, M
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2001, 42 (11) : 2450 - 2457
[25] B-cell clonality analysis by polymerase chain reaction (PCR) detection of immunoglobulin (Ig) κ and λ light chain gene rearrangements.
Chiu, A
Diss, T
Knowles, DM
Pan, LX
LABORATORY INVESTIGATION, 2001, 81 (01) : 159A - 159A
[26] RAPID CLONING OF REARRANGED IMMUNOGLOBULIN HEAVY-CHAIN GENES FROM HUMAN B-CELL LINES USING ANCHORED POLYMERASE CHAIN-REACTION
RATECH, H
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 182 (03) : 1260 - 1263
[27] Analysis of immunoglobulin heavy chain gene rearrangement in myoepithelial sialadenitis by polymerase chain reaction
Hsi, ED
Siddiqui, J
Schnitzer, B
Alkan, S
Ross, CW
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1996, 106 (04) : 498 - 503
[28] B-cell heavy chain gene rearrangement in classical Hodgkin lymphoma
Smith, L. B.
Schnitzer, B.
Weigelin, H. C.
Thorson, J. A.
MODERN PATHOLOGY, 2007, 20 : 261A - 261A
[29] B-Cell heavy chain gene rearrangement in classical Hodgkin lymphoma
Smith, L. B.
Schnitzer, B.
Weigelin, H. C.
Thorson, J. A.
LABORATORY INVESTIGATION, 2007, 87 : 261A - 261A
[30] Specificity of polymerase chain reaction-based clonality analysis of immunoglobulin heavy chain gene rearrangement for the detection of bone marrow infiltrate in B-cell lymphoma-associated haemophagocytic syndrome
Kojima, K
Kaneda, K
Yasukawa, M
Tanaka, K
Inoue, T
Yamashita, T
Dansako, H
Sakugawa, ST
Kozuka, T
Hara, M
Tanimoto, M
BRITISH JOURNAL OF HAEMATOLOGY, 2002, 119 (03) : 616 - 621

← 1 2 3 4 5 →