Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene

被引：14

作者：

Maezawa, Masaki ^{[1
,2
]}

Watanabe, Ken-ichi ^{[2
]}

Horiuchi, Noriyuki ^{[1
,2
]}

Matsumoto, Kotaro ^{[1
,2
]}

Kobayashi, Yoshiyasu ^{[1
,2
]}

Inokuma, Hisashi ^{[1
,2
]}

机构：

[1] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan

[2] Obihiro Univ Agr & Vet Med, Dept Vet Med, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 01期

关键词：

bovine B-cell lymphoma; clonality; polymerase chain reaction; PCR;

D O I：

10.1292/jvms.19-0418

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma.

引用

页码：61 / 63

页数：3

共 50 条

[1] Polymerase chain reaction detection of rearranged immunoglobulin heavy chain DNA in plasma samples is useful in the diagnosis of B-cell lymphoma
Tohda, S
Murakami, N
Nara, N
INTERNATIONAL JOURNAL OF HEMATOLOGY, 2000, 72 (01) : 74 - 78
[2] Evaluation of B-cell clonality in archival skin biopsy samples of cutaneous B-cell lymphoma by immunoglobulin heavy chain gene polymerase chain reaction
Lukowsky, A
Marchwat, M
Sterry, W
Gellrich, S
LEUKEMIA & LYMPHOMA, 2006, 47 (03) : 487 - 493
[3] Molecular diagnosis of B-cell Non Hodgkin lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement
Yousefian, A.
Poopak, B.
Abolghasemi, H.
Azarkeyvan, A.
Farhadi, M.
Farahani, K.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, 2008, 30 : 128 - 128
[4] Molecular analysis of the immunoglobulin heavy chain gene in the diagnosis of primary cutaneous B cell lymphoma
Child, FJ
Woolford, AJ
Calonje, E
Russell-Jones, R
Whittaker, SJ
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (04) : 984 - 989
[5] B-cell clonality determination using an immunoglobulin κ light chain polymerase chain reaction method
Pai, RK
Chakerian, AE
Binder, JM
Amin, M
Viswanatha, DS
JOURNAL OF MOLECULAR DIAGNOSTICS, 2005, 7 (02): : 300 - 307
[6] Diagnosis of conjunctival B-cell lymphoma by polymerase chain reaction heteroduplex analysis
Strauss, EC
Warren, JF
Margolis, TP
Holsclaw, DS
AMERICAN JOURNAL OF OPHTHALMOLOGY, 2003, 136 (01) : 207 - 209
[7] IMMUNOGLOBULIN HEAVY-CHAIN GENE REARRANGEMENT IN B-CELL NON-HODGKINS-LYMPHOMAS DETECTED BY THE POLYMERASE CHAIN-REACTION
ALAIBAC, M
PARADISO, A
GIANNELLA, C
LABRIOLA, A
MARZULLO, F
LORUSSO, V
DELENA, M
TUMORI, 1995, 81 (02) : 128 - 131
[8] Rapid diagnosis of clonal immunoglobulin heavy chain gene rearrangements in cutaneous B-Cell lymphomas using the LightCycler-polymerase chain reaction with DNA melting curve analysis
Xu, DS
Du, J
Kamino, H
Ratech, H
AMERICAN JOURNAL OF DERMATOPATHOLOGY, 2004, 26 (05) : 385 - 389
[9] Detection of monoclonality of the immunoglobulin heavy chain gene in thyroid malignant lymphoma by vectorette polymerase chain reaction
Takano, T
Miyauchi, A
Matsuzuka, F
Yoshida, H
Notomi, T
Kuma, K
Amino, N
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2005, 90 (02): : 720 - 723
[10] Sensitivity of an immunoglobulin heavy chain gene polymerase chain reaction primer system for routine diagnosis of lymphomas
R Heyny-von Haußen
C Braun
G Mall
Diagnostic Pathology, 2 (Suppl 1)

← 1 2 3 4 5 →