Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

被引：48
|
作者：
Han, Sewoon [1 ]
Yang, Kisuk [2 ]
Shin, Yoojin [1 ]
Lee, Jung Seung [2 ]
Kamm, Roger D. [3 ]
Chung, Seok [1 ]
Cho, Seung-Woo [2 ]
机构：
[1] Korea Univ, Dept Mech Engn, Seoul 136713, South Korea
[2] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea
[3] MIT, Dept Mech Engn, Cambridge, MA 02139 USA
来源：
LAB ON A CHIP | 2012年 / 12卷 / 13期
基金：
新加坡国家研究基金会;
关键词：
NEUROGENESIS; BEHAVIOR; RENEWAL; DESIGN; GROWTH;
D O I：
10.1039/c2lc21285d
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.
引用
收藏
页码：2305 / 2308
页数：4
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