DIFFERENTIATION OF NEURAL STEM CELLS REGULATED BY THREE-DIMENSIONAL TISSUE SHAPE

被引：0
|
作者：
Matsushiro, Yuki [1 ]
Kato-Negishi, Midori [2 ]
Onoe, Hiroaki [1 ]
机构：
[1] Keio Univ, Yokohama, Kanagawa, Japan
[2] Univ Tokyo, Inst Ind Sci, Tokyo, Japan
来源：
2017 19TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS) | 2017年
关键词：
Agarose hydrogel; Micropatterning; Stem cell; Differentiation induction;
D O I：
暂无
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.
引用
收藏
页码：285 / 288
页数：4
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