Optimization of artificial fertilization in the stinging catfish Heteropneustes fossilis (Bloch)

被引：1
|
作者：
Christopher, J. Godwin [1 ]
Murugesan, A. G. [2 ]
Sukumaran, N. [3 ]
机构：
[1] VIT Univ, Sch Biosci & Technol, Vellore 632014, Tamil Nadu, India
[2] Manonmaniam Sundaranar Univ, SPCES, Alwarkurichi 627412, India
[3] VELS Univ, Sch Life Sci, Madras 600117, Tamil Nadu, India
来源：
ZYGOTE | 2011年 / 19卷 / 01期
关键词：
Artificial breeding; Fertilization; Optimal sperm requirement; Sperm assessment; Sperm-to-egg ratio; TROUT SALMO-GAIRDNERI; SPERM; MOTILITY; QUALITY; SEMEN;
D O I：
10.1017/S0967199410000122
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Determination of optimum sperm requirement in artificial breeding helps to improve the fertilization efficiency, sperm economy and minimize the sacrificing of males for milt. Quantification of sperm cells was done by spectrophotometer at 420 nm followed by haemocytometer counting. Best correlation (r(2) = 0.97) was recorded. The minimum number of sperm required for optimal fertilization success in Heteropneustes fossilis was determined. An average fertilization success of 78-93% was recorded at 8 x 10(3) to 8 x 10(7) sperm per egg. The optimum contact duration of gametes was 5 minutes. The highest fertilization rate of 98.18% was recorded at 8 x 10(7) spermatozoa. egg(-1).
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页码：63 / 66
页数：4
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