Optimization of artificial fertilization in the stinging catfish Heteropneustes fossilis (Bloch)

被引:1
|
作者
Christopher, J. Godwin [1 ]
Murugesan, A. G. [2 ]
Sukumaran, N. [3 ]
机构
[1] VIT Univ, Sch Biosci & Technol, Vellore 632014, Tamil Nadu, India
[2] Manonmaniam Sundaranar Univ, SPCES, Alwarkurichi 627412, India
[3] VELS Univ, Sch Life Sci, Madras 600117, Tamil Nadu, India
关键词
Artificial breeding; Fertilization; Optimal sperm requirement; Sperm assessment; Sperm-to-egg ratio; TROUT SALMO-GAIRDNERI; SPERM; MOTILITY; QUALITY; SEMEN;
D O I
10.1017/S0967199410000122
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Determination of optimum sperm requirement in artificial breeding helps to improve the fertilization efficiency, sperm economy and minimize the sacrificing of males for milt. Quantification of sperm cells was done by spectrophotometer at 420 nm followed by haemocytometer counting. Best correlation (r(2) = 0.97) was recorded. The minimum number of sperm required for optimal fertilization success in Heteropneustes fossilis was determined. An average fertilization success of 78-93% was recorded at 8 x 10(3) to 8 x 10(7) sperm per egg. The optimum contact duration of gametes was 5 minutes. The highest fertilization rate of 98.18% was recorded at 8 x 10(7) spermatozoa. egg(-1).
引用
收藏
页码:63 / 66
页数:4
相关论文
共 50 条
  • [21] Effects of stress and food deprivation on catfish, Heteropneustes fossilis (Bloch)
    Sherwani, Fauzia A.
    Parwez, Iqbal
    Indian Journal of Experimental Biology, 2000, 38 (04) : 379 - 384
  • [22] ABNORMAL CORPUSCLE OF STANNIUS IN CATFISH, HETEROPNEUSTES-FOSSILIS (BLOCH)
    SUBHEDAR, N
    RAO, PDP
    CURRENT SCIENCE, 1974, 43 (17): : 550 - 551
  • [23] Optimisation of sperm cryopreservation protocol for Asian stinging catfish Heteropneustes fossilis (Bloch, 1794), to support hatchery seed production
    Lal, Kuldeep K.
    Barman, Anindya S.
    Punia, Peyush
    Khare, Praveen
    Mohindra, Vindhya
    Singh, Rajeev K.
    Sah, Rama S.
    Majhi, Sullip K.
    Lal, Bechan
    INDIAN JOURNAL OF FISHERIES, 2023, 70 (02): : 64 - 71
  • [24] TOXICITY OF CARBOFURAN TECHNICAL 75DB TO THE FERTILIZATION OF EGGS OF CATFISH, HETEROPNEUSTES-FOSSILIS (BLOCH)
    CHATTERJEE, S
    GHOSH, R
    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1995, 55 (01) : 111 - 115
  • [25] Toxicity of organophosphorus pesticide sumithion on larval stages of stinging catfish Heteropneustes fossilis
    Md.SHAHJAHAN
    Md.Farajul KABIR
    Kizar Ahmed SUMON
    Lipi Rani BHOWMIK
    Harunur RASHID
    Journal of Oceanology and Limnology, 2017, (01) : 109 - 114
  • [26] CESTODES (CARYOPHYLLIDEA) OF THE STINGING CATFISH HETEROPNEUSTES FOSSILIS (SILURIFORMES: HETEROPNEUSTIDAE) FROM ASIA
    Ash, Anirban
    Scholz, Tomas
    Oros, Mikulas
    Levron, Celine
    Kar, Pradip Kumar
    JOURNAL OF PARASITOLOGY, 2011, 97 (05) : 899 - 907
  • [27] Toxicity of organophosphorus pesticide sumithion on larval stages of stinging catfish Heteropneustes fossilis
    Md. Shahjahan
    Md. Farajul Kabir
    Kizar Ahmed Sumon
    Lipi Rani Bhowmik
    Harunur Rashid
    Chinese Journal of Oceanology and Limnology, 2017, 35 : 109 - 114
  • [28] Toxicity of organophosphorus pesticide sumithion on larval stages of stinging catfish Heteropneustes fossilis
    Shahjahan, Md.
    Kabir, Md. Farajul
    Sumon, Kizar Ahmed
    Bhowmik, Lipi Rani
    Rashid, Harunur
    CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2017, 35 (01): : 109 - 114
  • [29] POTENTIAL DANGER FROM INDIAN CATFISH, HETEROPNEUSTES-FOSSILIS (BLOCH)
    ROCHE, ET
    STAMANT, JA
    CALIFORNIA FISH AND GAME, 1973, 59 (02): : 144 - 145
  • [30] Dietary arginine requirement of fingerling Indian catfish (Heteropneustes fossilis, Bloch)
    Imtiaz Ahmed
    Aquaculture International, 2013, 21 : 255 - 271