LC-MS/MS method for the quantification of myo- and chiro-inositol as the urinary biomarkers of insulin resistance in human urine

被引：6
|
作者：
Kim, Byoung-Hyoun [1 ]
Park, Jin-Young [2 ]
Jang, Jae Bum [2 ]
Moon, Dong Cheul [3 ]
机构：
[1] LG Chem Ltd, Analyt Sci, Taejon 305380, South Korea
[2] Hoseo Univ, Reg Innovat Ctr, Baebang Eup 336795, Asan, South Korea
[3] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea
来源：
BIOMEDICAL CHROMATOGRAPHY | 2012年 / 26卷 / 04期
关键词：
myo-inositol; chiro-inositol; urinary biomarker; LC-MS; MS; method validation; LIQUID-CHROMATOGRAPHY; MYOINOSITOL;
D O I：
10.1002/bmc.1682
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple LC-MS/MS method has been developed and validated for the quantification of endogenous myo- and chiro-inositol in human urine. myo- and chiro-Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient (n?=?3) was greater than 0.9991 over the range 0.0525.0?mu g/mL with the weighted (1/C2) least square method. Precision (%RSD) and accuracy (%RE) were 010.0% and 06.0% for the intra-day assay (n?=?5) and 014.3% and 010.0% for the inter-day assay (n?=?5). myo- and chiro-Inositol have been shown to be stable in human urine stored at room temperature and for three freezethaw cycles. Copyright (c) 2011 John Wiley & Sons, Ltd.
引用
收藏
页码：429 / 433
页数：5
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