Validation of LC-MS/MS method for determination of ertapenem in human plasma and urine

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of ertapenem in human plasma and urine. After addition of internal standard (ceftazidime), plasma and urine was diluted with methanol and analyzed by LC-MS/MS. Using MS/ MS with multiple reaction monitoring (MRM) mode, ertapenem were selectively detected without severe interference from human plasma and urine. The standard calibration curve for ertapenem was linear (r(2) = 0.9996) over the concentration range 1 similar to 100 mu g/mL in human plasma. The intra-and inter-day precision over the concentration range of ertapenem was lower than 8.9% (correlation of variance, CV), and accuracy was between 97.2 similar to 106.2%. On the other hand, it was showed good relationship (r(2) = 0.9992) and the precision (intra-and inter-day) over the concentration range of ertapenem was lower than CV 7.2%, and accuracy was between 97.9 similar to 111.6% for urine. This method has been successfully applied to the pharmacokinetic study of ertapenem in human plasma and urine.