Molecular detection and seroprevalence of classical swine fever virus from 2016 to 2018 in pigs of Mizoram, India

Classical swine fever (CSF) is a fatal endemic disease of pig population of North eastern India in particular and India in general. Present study revealed molecular detection of CSFV and seroprevalence of the disease in pig population of Mizoram, India during 2016-2018. Serum samples from apparently healthy, unvaccinated pigs were collected in collaboration with the State Animal Husbandry and Veterinary Department, Mizoram and a total of 594 serum samples from 7 districts were subjected to detection of CSFV specific antibodies by indirect ELISA. A total of 206 (34.68%) serum samples were positive for CSFV antibodies by ELISA. District wise, Saiha district showed highest seroprevalence of the disease followed by Kolasib and Serchhip. Apart from this, during the same time period, CSFV suspected samples received in the Department of Veterinary Microbiology consisting of 269 serum samples, 10 whole blood and 83 tissue samples obtained from 8 districts of Mizoram were subjected to detection of NS5b and E-2 mRNA transcripts by nRT-PCR of which a total of 42 (11.60%) samples including serum (5.58%), tissues (27.71%) and whole blood (40%) were positive for the NS5b and E-2 mRNA transcripts, specific for CSFV. District wise analysis revealed that Aizawl has the highest percentage of positive samples of CSFV followed by Saiha and Lawngtlai district.