Inhibition of extracellular traps by spores of Trichoderma stromaticum on neutrophils obtained from human peripheral blood

被引:3
|
作者
Oliveira-Mendonca, Lucilla Silva [1 ]
Lopes, Danielle de Sousa [1 ]
Bozzi, Adriana [1 ,2 ]
Carvalho, Nani Oliveira [2 ]
Queiroz-Oliveira, Thamires [3 ]
Santos Matos, Melissa Ercilia [1 ]
de Carvalho, Andrea Teixeira [2 ]
Castro-Gomes, Thiago [3 ]
Mendes, Erica Araujo [4 ]
Augusto, Danillo G. [5 ,6 ]
Bispo Sousa, Sandra Mara [7 ]
Silva-Jardim, Izaltina [1 ]
Lima-Santos, Jane [1 ]
机构
[1] Univ Estadual Santa Cruz, Dept Ciencias Biol, Ilheus, BA, Brazil
[2] Fundacao Oswaldo Cruz, Inst Rene Rachou FIOCRUZ MINAS, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, Brazil
[4] Univ Sao Paulo, Inst Ciencias Biol, Dept Microbiol, Sao Paulo, Brazil
[5] Univ Fed Parana, Programa Posgrad Genet, Curitiba, Parana, Brazil
[6] Univ Calif San Francisco, Dept Neurol, San Francisco, CA USA
[7] Univ Estadual Sudoeste Bahia, Dept Ciencias Nat, Vitoria Da Conquista, Brazil
关键词
Trichoderma; Biocontrol agent; Neutrophils; NETs; microRNAs; MICRORNAS; MYCOPARASITE; GENOMICS;
D O I
10.1016/j.molimm.2021.11.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although the genus Trichoderma is widely used as a biocontrol agent in crops, little is known about its potential impact on the human immune system. In mice, our group has shown that exposition to T. asperelloides spores lead to reduced neutrophil counts in the peripheral blood and in the peritoneal cavity. In addition, T. stromaticum spores produced an inflammatory infiltrate on mice lungs, reducing the levels of IFN-gamma and IL-10 cytokines, reactive oxygen species, and receptors of microbial patterns. Here we demonstrate that the interaction of human peripheral neutrophils with T. stromaticum spores also leads to a reduced release of neutrophil extracellular traps (NETs) after induction with the NET-inducer agent phorbol 12-myristate 13-acetate. This interaction also reduced the expression levels of multiple microRNAs, such as miR-221, miR-222, miR-223 and miR-27a, as well as genes related to NETs, such as ELANE, MPO and PADI4. Furthermore, T. stromaticum spores affected the expression of the genes SOCS3, TLR4, CSNK2A1, GSDMD, and NFFKBIA, related to the activation of inflammatory immune responses in neutrophils. Overall, our results suggest T. stromaticum as a potential NET inhibitor and as an immunomodulatory agent. Since this fungus is used as biocontrol in crops, our findings point to the importance of advancing our knowledge on the effects of this bioagent on the human immune system. Finally, the study of the active compounds produced by the fungus is also important for the prospection of new drugs that could be used to block the exacerbation of inflammatory immune responses present in several human diseases.
引用
收藏
页码:43 / 52
页数:10
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