Inhibition of extracellular traps by spores of Trichoderma stromaticum on neutrophils obtained from human peripheral blood

被引:3
|
作者
Oliveira-Mendonca, Lucilla Silva [1 ]
Lopes, Danielle de Sousa [1 ]
Bozzi, Adriana [1 ,2 ]
Carvalho, Nani Oliveira [2 ]
Queiroz-Oliveira, Thamires [3 ]
Santos Matos, Melissa Ercilia [1 ]
de Carvalho, Andrea Teixeira [2 ]
Castro-Gomes, Thiago [3 ]
Mendes, Erica Araujo [4 ]
Augusto, Danillo G. [5 ,6 ]
Bispo Sousa, Sandra Mara [7 ]
Silva-Jardim, Izaltina [1 ]
Lima-Santos, Jane [1 ]
机构
[1] Univ Estadual Santa Cruz, Dept Ciencias Biol, Ilheus, BA, Brazil
[2] Fundacao Oswaldo Cruz, Inst Rene Rachou FIOCRUZ MINAS, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, Brazil
[4] Univ Sao Paulo, Inst Ciencias Biol, Dept Microbiol, Sao Paulo, Brazil
[5] Univ Fed Parana, Programa Posgrad Genet, Curitiba, Parana, Brazil
[6] Univ Calif San Francisco, Dept Neurol, San Francisco, CA USA
[7] Univ Estadual Sudoeste Bahia, Dept Ciencias Nat, Vitoria Da Conquista, Brazil
关键词
Trichoderma; Biocontrol agent; Neutrophils; NETs; microRNAs; MICRORNAS; MYCOPARASITE; GENOMICS;
D O I
10.1016/j.molimm.2021.11.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although the genus Trichoderma is widely used as a biocontrol agent in crops, little is known about its potential impact on the human immune system. In mice, our group has shown that exposition to T. asperelloides spores lead to reduced neutrophil counts in the peripheral blood and in the peritoneal cavity. In addition, T. stromaticum spores produced an inflammatory infiltrate on mice lungs, reducing the levels of IFN-gamma and IL-10 cytokines, reactive oxygen species, and receptors of microbial patterns. Here we demonstrate that the interaction of human peripheral neutrophils with T. stromaticum spores also leads to a reduced release of neutrophil extracellular traps (NETs) after induction with the NET-inducer agent phorbol 12-myristate 13-acetate. This interaction also reduced the expression levels of multiple microRNAs, such as miR-221, miR-222, miR-223 and miR-27a, as well as genes related to NETs, such as ELANE, MPO and PADI4. Furthermore, T. stromaticum spores affected the expression of the genes SOCS3, TLR4, CSNK2A1, GSDMD, and NFFKBIA, related to the activation of inflammatory immune responses in neutrophils. Overall, our results suggest T. stromaticum as a potential NET inhibitor and as an immunomodulatory agent. Since this fungus is used as biocontrol in crops, our findings point to the importance of advancing our knowledge on the effects of this bioagent on the human immune system. Finally, the study of the active compounds produced by the fungus is also important for the prospection of new drugs that could be used to block the exacerbation of inflammatory immune responses present in several human diseases.
引用
收藏
页码:43 / 52
页数:10
相关论文
共 50 条
  • [21] Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils
    Juliana D. B. Rocha
    Michelle T. C. Nascimento
    Debora Decote-Ricardo
    Suzana Côrte-Real
    Alexandre Morrot
    Norton Heise
    Marise P. Nunes
    José Osvaldo Previato
    Lucia Mendonça-Previato
    George A. DosReis
    Elvira M. Saraiva
    Célio G. Freire-de-Lima
    Scientific Reports, 5
  • [22] Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils
    Rocha, Juliana D. B.
    Nascimento, Michelle T. C.
    Decote-Ricardo, Debora
    Corte-Real, Suzana
    Morrot, Alexandre
    Heise, Norton
    Nunes, Marise P.
    Previato, Jose Osvaldo
    Mendonca-Previato, Lucia
    DosReis, George A.
    Saraiva, Elvira M.
    Freire-de-Lima, Celio G.
    SCIENTIFIC REPORTS, 2015, 5 : 8008
  • [23] Nitric oxide donors release extracellular traps from human neutrophils by augmenting free radical generation
    Patel, Satyananda
    Kumar, Sachin
    Jyoti, Anupam
    Srinag, Bangalore Suresh
    Keshari, Ravi Shankar
    Saluja, Rohit
    Verma, Anupam
    Mitra, Kalyan
    Barthwal, Manoj Kumar
    Krishnamurthy, Hanumanthappa
    Bajpai, Virendra K.
    Dikshit, Madhu
    NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2010, 22 (03): : 226 - 234
  • [24] Mevalonate-dependent inhibition of transendothelial migration and chemotaxis of human peripheral blood neutrophils by pravastatin
    Dunzendorfer, S
    Rothbucher, D
    Schratzberger, P
    Reinisch, N
    Kahler, CM
    Wiedermann, CJ
    CIRCULATION RESEARCH, 1997, 81 (06) : 963 - 969
  • [25] Blood-brain crosstalk: the roles of neutrophils, platelets, and neutrophil extracellular traps in neuropathologies
    Chou, Ming-Li
    Babamale, Abdulkareem Olarewaju
    Walker, Tara L.
    Cognasse, Fabrice
    Blum, David
    Burnouf, Thierry
    TRENDS IN NEUROSCIENCES, 2023, 46 (09) : 764 - 779
  • [26] Neutrophils release extracellular DNA traps during storage of red blood cell units
    Fuchs, Tobias A.
    Alvarez, Javier J.
    Martinod, Kimberly
    Bhandari, Ashish A.
    Kaufman, Richard M.
    Wagner, Denisa D.
    TRANSFUSION, 2013, 53 (12) : 3210 - 3216
  • [27] Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood
    Cui, Chang
    Schoenfelt, Kelly Q.
    Becker, Kristen M.
    Becker, Lev
    STAR PROTOCOLS, 2021, 2 (04):
  • [28] REACTIVITY OF CIRCULATING HUMAN PERIPHERAL-BLOOD NEUTROPHILS
    BAKUEV, MM
    ZURKHAEVA, RZ
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 1992, 114 (11) : 1714 - 1717
  • [29] Neutrophils Extracellular Traps Inhibition Improves PD-1 Blockade Immunotherapy in Colorectal Cancer
    Zhang, Hongji
    Wang, Yu
    Onuma, Amblessed
    He, Jiayi
    Wang, Han
    Xia, Yujia
    Lal, Rhea
    Cheng, Xiang
    Kasumova, Gyulnara
    Hu, Zhiwei
    Deng, Meihong
    Beane, Joal D.
    Kim, Alex C.
    Huang, Hai
    Tsung, Allan
    CANCERS, 2021, 13 (21)
  • [30] Heparin induces apoptosis in human peripheral blood neutrophils
    Manaster, J
    Chezar, J
    ShurtzSwirski, R
    Shapiro, G
    Tendler, Y
    Kristal, B
    Shasha, SM
    Sela, S
    BRITISH JOURNAL OF HAEMATOLOGY, 1996, 94 (01) : 48 - 52