A non-isotopic method for the detection of telomerase activity in tumour tissues: TRAP-silver staining assay

被引：26

作者：

Wen, JM ^{[1
]}

Sun, LB

Zhang, M

Zheng, MH

机构：

[1] Sun Yat Sen Univ Med Sci, Dept Pathol, Guangzhou 510089, Peoples R China

[2] Univ Western Australia, Dept Orthopaed Surg, Nedlands, WA 6009, Australia

来源：

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 1998年 / 51卷 / 02期

关键词：

telomerase; TRAP; bone tumours; silver staining;

D O I：

10.1136/mp.51.2.110

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Telomerase activity has been observed in a high proportion of specimens from a wide variety of human malignant tumours, but not in non-neoplastic tissues. Measurement of telomerase activity is a possible diagnostic maker for malignant tumours. A non-isotopic telomeric repeat amplification protocol (TRAP) silver staining method for the detection of telomerase activity in human bone tumour tissues is described. The TRAP-silver staining method was quick, safe, and effective, and can be used as a routine diagnostic method for the detection of telomerase activity in bone tumours.

引用

页码：110 / 112

页数：3

共 48 条

[41] Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
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