A non-isotopic method for the detection of telomerase activity in tumour tissues: TRAP-silver staining assay

被引：26

作者：

Wen, JM ^{[1
]}

Sun, LB

Zhang, M

Zheng, MH

机构：

[1] Sun Yat Sen Univ Med Sci, Dept Pathol, Guangzhou 510089, Peoples R China

[2] Univ Western Australia, Dept Orthopaed Surg, Nedlands, WA 6009, Australia

来源：

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 1998年 / 51卷 / 02期

关键词：

telomerase; TRAP; bone tumours; silver staining;

D O I：

10.1136/mp.51.2.110

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Telomerase activity has been observed in a high proportion of specimens from a wide variety of human malignant tumours, but not in non-neoplastic tissues. Measurement of telomerase activity is a possible diagnostic maker for malignant tumours. A non-isotopic telomeric repeat amplification protocol (TRAP) silver staining method for the detection of telomerase activity in human bone tumour tissues is described. The TRAP-silver staining method was quick, safe, and effective, and can be used as a routine diagnostic method for the detection of telomerase activity in bone tumours.

引用

页码：110 / 112

页数：3

共 48 条

[31] A novel method to detect clinically localized prostate cancer: Telomerase repeat amplification protocol (TRAP) assay detects telomerase activity in prostatic secretions.
Ramin, SA
Wang, ZL
Ruckle, HC
Lui, PD
Beltz, R
Kyeyune-Nyombi, E
Sands, J
JOURNAL OF UROLOGY, 1998, 159 (05): : 180 - 180
[32] A quantitative method to measure telomerase activity by hybridization protectin assay (HPA) connected with TRAP for the diagnosis of hepatocellular carcinoma (HCC).
Takaishi, H
Nakashio, R
Kitamoto, M
Takahashi, S
Nakanishi, T
Tahara, H
Ide, T
Asada, N
Aikata, H
Kamata, K
Kawakami, Y
Kajiyama, G
GASTROENTEROLOGY, 1998, 114 (04) : A1351 - A1351
[33] Loss of heterozygosity of the TP53 tumor suppressor gene and detection of point mutations by the non-isotopic RNAse cleavage assay in prostate cancer
Macera, MJ
Godec, CJ
Sharma, N
Verma, RS
CANCER GENETICS AND CYTOGENETICS, 1999, 108 (01) : 42 - 47
[34] Detection of telomerase activity in human carcinomas using a TRAP-ELISA method:: Correlation with hTR and hTERT expression
González-Quevedo, R
De Juan, C
Massa, MJ
Sánchez-Pernaute, A
Torres, A
Balibrea, JL
Benito, M
Iniesta, P
INTERNATIONAL JOURNAL OF ONCOLOGY, 2000, 16 (03) : 623 - 628
[35] A FULLY AUTOMATED NON-ISOTOPIC METHOD FOR THE ASSAY OF HAPTENS, ANTIGENS AND ANTIBODIES AS APPLIED TO FERRITIN, ALPHA-FETOPROTEIN, IGG AND T4
HOLY, HW
COLLETCASSARD, D
MAGNUSSON, CG
MASSON, PL
JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 1980, 18 (10): : 701 - 701
[36] A non-isotopic method for the determination of activity of the thermostable NAD-dependent DNA ligase from Thermus thermophilus HB8
Muerhoff, AS
Dawson, GJ
Desai, SM
JOURNAL OF VIROLOGICAL METHODS, 2004, 119 (02) : 171 - 176
[37] Simple non-isotopic method for detection of lipoprotein lipase (LPL) ASN291->ser mutation and screening or a selected hypertriglyceridemic population.
Carmody, MB
Massanari, D
Rundell, CA
Keilson, LM
Vary, CPH
AMERICAN JOURNAL OF PATHOLOGY, 1996, 149 (05): : G13 - G13
[38] Fast and reliable mutation detection of the complete exon 11-15 JAK2 coding region using non-isotopic RNase cleavage assay (NIRCA)
Kambas, Konstantinos
Mitroulis, Ioannis
Kourtzelis, Ioannis
Chrysanthopoulou, Akrivi
Speletas, Matthaios
Ritis, Konstantinos
EUROPEAN JOURNAL OF HAEMATOLOGY, 2009, 83 (03) : 215 - 219
[39] Extremely rapid non-isotopic PCR-based method for the detection of IgH and bcl-1 gene rearrangements in mantle cell lymphoma (MCL).
Segal, GH
Maiese, RL
LABORATORY INVESTIGATION, 1996, 74 (01) : 712 - 712
[40] Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells
Fan, X
Furnari, FB
Cavenee, WK
Castresana, JS
INTERNATIONAL JOURNAL OF ONCOLOGY, 2001, 18 (05) : 1023 - 1026

← 1 2 3 4 5 →