Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats

被引：7
|
作者：
Schnell, J. A. [1 ]
Treyvaud-Amiguet, V. [1 ]
Arnason, J. T. [1 ]
Johnson, D. A. [1 ]
机构：
[1] Univ Ottawa, Ottawa Carleton Inst Biol, Stn A, Ottawa, ON K1N 6N5, Canada
来源：
TRANSGENIC RESEARCH | 2012年 / 21卷 / 04期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Soybean seed coat; Metabolic engineering; Bioplastics; Polyhydroxybutyrate; POLY-BETA-HYDROXYBUTYRATE; ALCALIGENES-EUTROPHUS H16; TRANSGENIC PLANTS; BIOSYNTHETIC-PATHWAY; ESCHERICHIA-COLI; PEROXIDASE; GENE; KETOTHIOLASE; PROTEIN; CLONING;
D O I：
10.1007/s11248-011-9575-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The feasibility of genetically engineering soybean seed coats to divert metabolism towards the production of novel biochemicals was tested. The genes phbA, phbB, phbC from Ralstonia eutropha each under the control of the seed coat peroxidase promoter were introduced into soybean and the production of polyhydroxybutyrate (PHB) was assayed. The analysis of seed coats arising from 4 independent transformation events demonstrated that PHB was produced at a mean of 0.12% seed coat dried weight with individual values up to 0.36%. These values demonstrate that it is possible to metabolically engineer soybean seed coats.
引用
收藏
页码：895 / 899
页数：5
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