Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats

被引：7
|
作者：
Schnell, J. A. [1 ]
Treyvaud-Amiguet, V. [1 ]
Arnason, J. T. [1 ]
Johnson, D. A. [1 ]
机构：
[1] Univ Ottawa, Ottawa Carleton Inst Biol, Stn A, Ottawa, ON K1N 6N5, Canada
来源：
TRANSGENIC RESEARCH | 2012年 / 21卷 / 04期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Soybean seed coat; Metabolic engineering; Bioplastics; Polyhydroxybutyrate; POLY-BETA-HYDROXYBUTYRATE; ALCALIGENES-EUTROPHUS H16; TRANSGENIC PLANTS; BIOSYNTHETIC-PATHWAY; ESCHERICHIA-COLI; PEROXIDASE; GENE; KETOTHIOLASE; PROTEIN; CLONING;
D O I：
10.1007/s11248-011-9575-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The feasibility of genetically engineering soybean seed coats to divert metabolism towards the production of novel biochemicals was tested. The genes phbA, phbB, phbC from Ralstonia eutropha each under the control of the seed coat peroxidase promoter were introduced into soybean and the production of polyhydroxybutyrate (PHB) was assayed. The analysis of seed coats arising from 4 independent transformation events demonstrated that PHB was produced at a mean of 0.12% seed coat dried weight with individual values up to 0.36%. These values demonstrate that it is possible to metabolically engineer soybean seed coats.
引用
收藏
页码：895 / 899
页数：5
相关论文
共 50 条
  • [1] Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats
    J. A. Schnell
    V. Treyvaud-Amiguet
    J. T. Arnason
    D. A. Johnson
    Transgenic Research, 2012, 21 : 895 - 899
  • [2] Expression of genes SBP and leginsulin in contrasting soybean seed coats
    Bahry, Carlos Andre
    Zimmer, Paulo Dejalma
    CIENCIA RURAL, 2016, 46 (10): : 1695 - 1700
  • [3] Relative expression of CYP and Arabinogalactan genes in soybean seed coats
    Bahry, Carlos Andre
    Venske, Eduardo
    Perboni, Anelise Tessari
    Zimmer, Paulo Dejalma
    REVISTA CIENCIA AGRONOMICA, 2018, 49 (01): : 78 - 84
  • [4] Metabolic engineering to increase isoflavone biosynthesis in soybean seed
    Yu, O
    Shi, J
    Hession, AO
    Maxwell, CA
    McGonigle, B
    Odell, JT
    PHYTOCHEMISTRY, 2003, 63 (07) : 753 - 763
  • [5] INHERITANCE OF PEROXIDASE ACTIVITY IN SOYBEAN SEED COATS
    BUZZELL, RI
    BUTTERY, BR
    CROP SCIENCE, 1969, 9 (03) : 387 - &
  • [6] PIGMENTATION OF SOYBEAN SEED COATS ASSOCIATED WITH BACTERIA
    DUNLEAVY, J
    PHYTOPATHOLOGY, 1962, 52 (08) : 730 - +
  • [7] Programmed cell death in soybean seed coats
    Rocha, Gustavo Lemos
    Pireda, Saulo
    Araujo, Jucelia da Silva
    Amancio Oliveira, Antonia Elenir
    Tavares Machado, Olga Lima
    da Cunha, Maura
    Grativol, Clicia
    Sales Fernandes, Katia Valevski
    PLANT SCIENCE, 2019, 288
  • [8] WATER TRANSPORT ACROSS SEED COATS OF SOYBEAN
    Fengshan, M.
    Meyer, C. J.
    Peterson, C. A.
    ACTA PHYSIOLOGIAE PLANTARUM, 2004, 26 (03) : 78 - 78
  • [9] Metabolic engineering of soybean affords improved phytosterol seed traits
    Neelakandan, Anjanasree K.
    Chamala, Swetha
    Valliyodan, Babu
    Nes, William David
    Nguyen, Henry T.
    PLANT BIOTECHNOLOGY JOURNAL, 2012, 10 (01) : 12 - 19
  • [10] DEVELOPMENT AND EVALUATION OF IMPERMEABLE SEED COATS FOR PRESERVING SOYBEAN SEED QUALITY
    HARTWIG, EE
    POTTS, HC
    CROP SCIENCE, 1987, 27 (03) : 506 - 508
12345